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          Sci-rna-seq3
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          EFO:0030028
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                Single-cell combinatorial indexing (‘sci-’) is a methodological framework that employs split-pool barcoding to uniquely label the nucleic acid contents of large numbers of single cells or nuclei. The sci-RNA-seq3 is an updated version of sci-RNA-seq. The major improvements are: (1) nuclei are extracted directly from fresh tissues without enzymatic treatment; (2) hairpin ligation for the third level indexing (barcoded Tn5 tagmentation was used in the previous version); (3) individually optimised enzymatic reactions; (4) FACS was replaced by dilution, and sonication and filtration steps were added to minimize aggregation.
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              <a target="_blank" class="inline-flex items-center px-2.5 py-1.5 text-xs font-medium rounded text-gray-700 bg-gray-100 hover:bg-gray-200" href="http://purl.obolibrary.org/obo/EFO_0030028">
                View in Ontology Tree
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