Homo sapiens

Effector cd8-positive, alpha-beta t cell Granulocyte Cd1c-positive myeloid dendritic cell Natural killer cell Mature nk t cell Cd141-positive myeloid dendritic cell Classical monocyte Plasmacytoid dendritic cell Effector cd4-positive, alpha-beta t cell Plasma cell Cd8-positive, alpha-beta thymocyte Cd4-positive, alpha-beta thymocyte B cell Dendritic cell Non-classical monocyte

Transition zone of prostate Peripheral zone of prostate

71-year-old stage 29-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 67-year-old stage 31-year-old stage 73-year-old stage

Benign prostatic hyperplasia Normal

Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Cd8-positive, alpha-beta t cell Exhausted t cell Cd4-positive helper t cell Regulatory t cell Myeloid cell Plasma cell Malignant cell T follicular helper cell B cell

Lymph node

Adult stage

Metastatic melanoma

Slide-seqv2

Homo sapiens

T cell Natural killer cell Cytotoxic t cell Regulatory t cell

Prostate gland

69-year-old stage 75-year-old stage 71-year-old stage 61-year-old stage 54-year-old stage 68-year-old stage 72-year-old stage 59-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 63-year-old stage

Prostatic acinar adenocarcinoma

Male

10x 3' v3

Cell

Homo sapiens

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Cortex of kidney

65-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

T cell Neutrophil Ciliated cell Pulmonary alveolar epithelial cell Endothelial cell Mast cell Macrophage Monocyte Fibroblast

Tuberculosis Cancer

Seq-well

Homo sapiens

L6b glutamatergic cortical neuron

Dorsolateral prefrontal cortex

69-year-old stage 75-year-old stage 42-year-old stage 89-year-old stage 87-year-old stage 85-year-old stage 88-year-old stage 68-year-old stage 29-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 80-year-old stage 72-year-old stage 81-year-old stage 80 year-old and over stage 77-year-old stage 86-year-old stage 70-year-old stage 78-year-old stage 83-year-old stage

Normal Dementia

Female Male

10x multiome 10x 3' v3

Nucleus

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Autosomal dominant polycystic kidney disease

Male

Slide-seqv2

Homo sapiens

Astrocyte

Prefrontal cortex White matter of temporal lobe White matter of frontal lobe White matter of occipital lobe White matter of parietal lobe

53-year-old stage 68-year-old stage 82-year-old stage 72-year-old stage 81-year-old stage 70-year-old stage 76-year-old stage 83-year-old stage

Normal Alzheimer disease Leukoencephalopathy, diffuse hereditary, with spheroids 1

Female Male

10x 3' v3

Nucleus

Homo sapiens

Near-projecting glutamatergic cortical neuron

Brodmann (1909) area 25 Lateral amygdaloid nucleus Temporal fusiform gyrus Brodmann (1909) area 19 Primary visual cortex Claustrum of brain Inferior temporal gyrus Subicular complex Piriform cortex Hippocampal field Brodmann (1909) area 13 Middle temporal gyrus Central amygdaloid nucleus Septal nuclear complex Extrastriate cortex Brodmann (1909) area 38 Lateral entorhinal cortex Supraoptic nucleus Brodmann (1909) area 32 Midbrain tectum Brodmann (1909) area 40 Inferior frontal gyrus Agranular insular cortex Brodmann (1909) area 43 Nucleus accumbens Substantia innominata Anterior cortical amygdaloid nucleus Primary somatosensory cortex Brodmann (1909) area 14 Anterior olfactory nucleus Accessory basal amygdaloid nucleus Primary auditory cortex Hypothalamus Brodmann (1909) area 23 Primary motor cortex Brodmann (1909) area 24 Brodmann (1909) area 46 Medial zone of hypothalamus Posterior parahippocampal gyrus Perirhinal cortex Granular insular cortex Retrosplenial region Cuneus cortex Lateral posterior nucleus of thalamus Superior temporal gyrus Bed nucleus of stria terminalis Superior parietal cortex Preoptic area

42-year-old stage 29-year-old stage 50-year-old stage 60-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Cd8-positive, alpha-beta t cell Cd4-positive helper t cell Epithelial cell Regulatory t cell Myeloid cell Plasma cell Malignant cell B cell Fibroblast

Lung

61-year-old stage

Renal cell carcinoma

Male

Slide-seqv2

Homo sapiens

Neutrophil Blood vessel endothelial cell Natural killer cell Cd8-positive, alpha-beta t cell Cd4-positive helper t cell Ionocyte Myoepithelial cell Macrophage Mural cell Keratinocyte Acinar cell of salivary gland Plasma cell Salivary gland glandular cell B cell Fibroblast Dendritic cell

Submandibular gland Parotid gland

69-year-old stage Sixth decade stage 48-year-old stage 59-year-old stage 62-year-old stage Fourth decade stage

Normal

Female Male

10x 3' v3

Cell

Homo sapiens

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Renal medulla

65-year-old stage

Normal

Male

Slide-seqv2