Homo sapiens

Endothelial cell Melanocyte Leukocyte Skin fibroblast Keratinocyte Pericyte

Skin of body Dermis Skin epidermis

65-year-old stage 39-year-old stage 56-year-old stage 40-year-old stage

Normal

Female Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell

Renal medulla

62-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell

Renal medulla

54-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

T cell Glutamatergic neuron Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Tac3-positive striatal interneuron Microglial cell Gabaergic interneuron Mural cell Pvalb gabaergic interneuron Astrocyte Sst gabaergic interneuron

Amygdala

52-year-old stage 54-year-old stage 68-year-old stage 51-year-old stage 59-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Normal

Male

Slide-seqv2

Mus musculus

Tracheal epithelial cell

Tracheal epithelial cell

Mature stage

Normal

10x 3' v3

Cell

Mus musculus

Megakaryocyte

Embryo

Theiler stage 22 Theiler stage 27 Theiler stage 24 Theiler stage 18 Theiler stage 25 Theiler stage 14 Theiler stage 16 Theiler stage 17 Theiler stage 15 Theiler stage 19 Theiler stage 26 Theiler stage 13 Theiler stage 20 Theiler stage 12 Theiler stage 21 Theiler stage 23

Normal

Female Male

Sci-rna-seq3

Nucleus

Drosophila melanogaster

Mechanosensory neuron of leg chordotonal organ Gustatory receptor neuron Hemocyte Epithelial cell Muscle cell Mechanosensory neuron Sensory neuron Ascending mechanosensory neuron of leg chordotonal organ

Leg

Day 5 of adulthood Day 2 of adulthood

Normal

Female Male

10x 3' v3

Homo sapiens

Cd8-positive, alpha-beta t cell Cd4-positive helper t cell Epithelial cell Regulatory t cell Myeloid cell Plasma cell Malignant cell B cell Fibroblast

Lung

61-year-old stage

Renal cell carcinoma

Male

Slide-seqv2

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Preoptic area

50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Natural killer cell Gamma-delta t cell Cd4-positive helper t cell Natural t-regulatory cell Activated cd8-positive, alpha-beta t cell Memory t cell

Duodenum Ileum Colon Submucosal esophageal gland Body of stomach Cardia of stomach Rectum Esophagogastric junction Lower esophagus

66-year-old stage 69-year-old stage 34-year-old stage 42-year-old stage 43-year-old stage 53-year-old stage 68-year-old stage 48-year-old stage 29-year-old stage 56-year-old stage 55-year-old stage 50-year-old stage 51-year-old stage 57-year-old stage 74-year-old stage 80 year-old and over stage 58-year-old stage 67-year-old stage 77-year-old stage 45-year-old stage 62-year-old stage 41-year-old stage 63-year-old stage 83-year-old stage 27-year-old stage

Barrett esophagus Normal Gastric intestinal metaplasia Gastritis

Female Male

10x 3' v3 10x 3' v2

Cell

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2