Homo sapiens

Oligodendrocyte precursor cell Oligodendrocyte Committed oligodendrocyte precursor Schwann cell

Medulla oblongata Hindbrain Telencephalon Midbrain Cerebellum Brain Diencephalon Pons

13th week post-fertilization stage 12th week post-fertilization stage 9th week post-fertilization stage 10th week post-fertilization stage Embryonic stage

Normal

Female Male

10x multiome

Nucleus

Homo sapiens

L5 extratelencephalic projecting glutamatergic cortical neuron

Dorsolateral prefrontal cortex

69-year-old stage 75-year-old stage 42-year-old stage 89-year-old stage 87-year-old stage 85-year-old stage 88-year-old stage 68-year-old stage 29-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 80-year-old stage 72-year-old stage 81-year-old stage 80 year-old and over stage 77-year-old stage 86-year-old stage 70-year-old stage 78-year-old stage 83-year-old stage

Normal Dementia

Female Male

10x multiome 10x 3' v3

Nucleus

Mus musculus

Endothelial cell Thymocyte Macrophage Dn4 thymocyte Epithelial cell of thymus Fibroblast

Thymus

3-month-old stage 20-month-old stage and over 18-month-old stage

Normal

Female Male

Smart-seq2

Cell

Homo sapiens

Central memory cd4-positive, alpha-beta t cell Cycling t cell Natural killer cell Cytotoxic t cell Mature nk t cell Effector memory cd4-positive, alpha-beta t cell Lymphocyte T-helper 17 cell Regulatory t cell Effector t cell Plasma cell B cell Effector memory cd8-positive, alpha-beta t cell

Apex of heart Heart left ventricle

21-year-old stage 26-year-old stage Sixth decade stage 61-year-old stage 52-year-old stage 30-year-old stage 29-year-old stage 38-year-old stage Fifth decade stage 55-year-old stage 28-year-old stage Third decade stage 49-year-old stage 24-year-old stage Seventh decade stage 25-year-old stage 32-year-old stage 64-year-old stage 70-year-old stage 31-year-old stage 19-year-old stage Eighth decade stage 27-year-old stage

Myocarditis Normal

Female Male

10x 3' v3 10x 3' v2

Nucleus

Mus musculus

Cardiac valve cell Spinal cord interneuron Premigratory neural crest cell Paraxial cell Notochordal cell Neuroplacodal cell Neurectodermal cell Hemangioblast Mesodermal cell Gut endothelial cell Primitive red blood cell Splanchnic mesodermal cell Surface ectodermal cell Midbrain dopaminergic neuron Endodermal cell Anterior visceral endoderm cell

Embryo

Theiler stage 14

Normal

Slide-seqv2

Homo sapiens

Kupffer cell Inflammatory macrophage

Caudate lobe of liver

Prime adult stage Young adult stage

Normal

Female Male

10x 3' v3 10x 3' v2

Cell Nucleus

Homo sapiens

Neural cell

Heart right ventricle Right cardiac atrium Apex of heart Heart left ventricle Left cardiac atrium Interventricular septum

Sixth decade stage Fifth decade stage Seventh decade stage Eighth decade stage

Normal

Female Male

10x 3' v3 10x 3' v2

Nucleus Cell

Homo sapiens

Endothelial cell of artery Capillary endothelial cell Vein endothelial cell

Duodenum Colon Submucosal esophageal gland Body of stomach Cardia of stomach Rectum Esophagogastric junction Lower esophagus

69-year-old stage 34-year-old stage 43-year-old stage 53-year-old stage 54-year-old stage 68-year-old stage 48-year-old stage 29-year-old stage 56-year-old stage 55-year-old stage 50-year-old stage 51-year-old stage 57-year-old stage 74-year-old stage 80 year-old and over stage 58-year-old stage 67-year-old stage 77-year-old stage 45-year-old stage 62-year-old stage 41-year-old stage 63-year-old stage 83-year-old stage 27-year-old stage

Barrett esophagus Normal Gastric intestinal metaplasia Gastritis

Female Male

10x 3' v3 10x 3' v2

Cell

Mus musculus

Tracheal epithelial cell

Tracheal epithelial cell

Mature stage

Normal

10x 3' v3

Cell

Homo sapiens

Microglial cell

Neocortex

Adult stage

Normal

Female Male

10x 3' v3

Cell

Mus musculus

Cardiac valve cell Spinal cord interneuron Premigratory neural crest cell Paraxial cell Notochordal cell Neuroplacodal cell Neurectodermal cell Hemangioblast Mesodermal cell Gut endothelial cell Primitive red blood cell Splanchnic mesodermal cell Surface ectodermal cell Midbrain dopaminergic neuron Endodermal cell Anterior visceral endoderm cell

Embryo

Theiler stage 14

Normal

Slide-seqv2

Mus musculus

Cardiac valve cell Spinal cord interneuron Premigratory neural crest cell Paraxial cell Notochordal cell Neuroplacodal cell Neurectodermal cell Hemangioblast Mesodermal cell Gut endothelial cell Primitive red blood cell Splanchnic mesodermal cell Surface ectodermal cell Midbrain dopaminergic neuron Endodermal cell Anterior visceral endoderm cell

Embryo

Theiler stage 14

Normal

Slide-seqv2