Homo sapiens

Podocyte Renal principal cell Parietal epithelial cell Renal beta-intercalated cell Endothelial cell Leukocyte Epithelial cell of proximal tubule Renal alpha-intercalated cell Kidney distal convoluted tubule epithelial cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney loop of henle thin ascending limb epithelial cell Fibroblast

Cortex of kidney

61-year-old stage 52-year-old stage 54-year-old stage 50-year-old stage 51-year-old stage 57-year-old stage 74-year-old stage 59-year-old stage 62-year-old stage 78-year-old stage

Normal Type 2 diabetes mellitus

Female Male

10x 5' v1 10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell B cell Pericyte Cerebral cortex neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron Hippocampal ca4 neuron

Uncal ca1

50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Brodmann (1909) area 40

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell B cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Granular insular cortex

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Oligodendrocyte precursor cell

Cerebral cortex

6-month-old stage 4-week-old stage 12-month-old stage 24-month-old stage

Normal

Female Male

10x 3' v3

Cell

Homo sapiens

T cell Neutrophil Natural killer cell Endothelial cell of lymphatic vessel Endothelial cell Mast cell Cd8-positive, alpha-beta t cell Basal cell Schwann cell Taste receptor cell Myeloid dendritic cell Myoepithelial cell Macrophage Regulatory t cell Mural cell Tongue muscle cell Plasma cell Cd4-positive, alpha-beta t cell B cell Monocyte Stratified squamous epithelial cell Fibroblast Salivary gland cell

Anterior part of tongue Tongue Posterior part of tongue

69-year-old stage 38-year-old stage 56-year-old stage 59-year-old stage 60-year-old stage

Normal

Female Male

Smart-seq2 10x 3' v3

Cell

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Pericyte Cerebral cortex neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Corticomedial nuclear complex

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Endothelial cell of venule Mast cell Schwann cell Melanocyte Lymphocyte Myeloid cell Keratinocyte Hair follicle cell Plasma cell Langerhans cell B cell Vascular associated smooth muscle cell Fibroblast Sebocyte

Disease Alopecia Leprosy Psoriasis Acne Granuloma annulare

Seq-well

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Inferior frontal gyrus

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

L2/3 intratelencephalic projecting glutamatergic neuron L5/6 near-projecting glutamatergic neuron Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte L4 intratelencephalic projecting glutamatergic neuron Cerebral cortex gabaergic interneuron Microglial cell Vip gabaergic interneuron Pvalb gabaergic interneuron Cerebral cortex neuron Astrocyte Sst gabaergic interneuron

Medial orbital frontal cortex

66-year-old stage 69-year-old stage 68-year-old stage 55-year-old stage 82-year-old stage 90-year-old stage 57-year-old stage 58-year-old stage 77-year-old stage 60-year-old stage 78-year-old stage 63-year-old stage 79-year-old stage 83-year-old stage

Covid-19 Breast cancer Chronic obstructive pulmonary disease Cardiomyopathy Myocardial infarction Influenza Small cell lung carcinoma Tongue cancer Heart disorder

Female Male

10x 3' v3

Nucleus

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Cerebellar inhibitory gabaergic interneuron Ependymal cell Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Substantia innominata

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Primary somatosensory cortex

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus