Homo sapiens

T cell Nasal mucosa goblet cell Club cell Keratinizing barrier epithelial cell Ionocyte Respiratory tract epithelial cell Respiratory basal cell Keratinocyte Glandular secretory epithelial cell Professional antigen presenting cell Respiratory tract multiciliated cell

Nasal cavity epithelium

66-year-old stage 34-year-old stage 65-year-old stage 30-year-old stage 59-year-old stage 64-year-old stage 63-year-old stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

T cell Natural killer cell Activated cd4-positive, alpha-beta t cell Mast cell Cd8-alpha-beta-positive, alpha-beta intraepithelial t cell Intestinal tuft cell Enteroendocrine cell Goblet cell Transit amplifying cell of colon Macrophage Intestinal crypt stem cell Follicular b cell Innate lymphoid cell Regulatory t cell Enterocyte Plasma cell Cd4-positive, alpha-beta t cell Cd8-positive, alpha-beta thymocyte Cd4-positive, alpha-beta memory t cell B cell Fibroblast Dendritic cell M cell of gut

Caecum epithelium Colonic epithelium Mucosa of transverse colon

Adult stage

Normal

Female Male

10x 3' v1 10x 3' v2

Cell

Homo sapiens

T cell Natural killer cell Retinal ganglion cell Retinal pigment epithelial cell Mast cell Off-bipolar cell Melanocyte On-bipolar cell Rod bipolar cell Endothelial cell of vascular tree Microglial cell Retinal cone cell Retinal rod cell Retina horizontal cell Amacrine cell Pericyte Mueller cell Monocyte Astrocyte Fibroblast

Pigment epithelium of eye Retina

Adult stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Vascular leptomeningeal cell Macroglial cell Astrocyte

Brodmann (1909) area 19 Brodmann (1909) area 24 Brodmann (1909) area 25 Brodmann (1909) area 38 Brodmann (1909) area 46 Agranular insular cortex Amygdala Anterior cingulate cortex Basolateral amygdaloid nuclear complex Bed nucleus of stria terminalis Body of caudate nucleus Central amygdaloid nucleus Central nucleus of inferior colliculus Cerebellum Claustrum of brain Corticomedial nuclear complex Cuneus cortex Dentate gyrus of hippocampal formation Globus pallidus Granular insular cortex Hippocampal field Hippocampal formation Inferior temporal gyrus Insula Lateral entorhinal cortex Medial dorsal nucleus of thalamus Medial entorhinal cortex Middle temporal gyrus Nucleus accumbens Parietal cortex Piriform cortex Pontine nuclear group Posterior parahippocampal gyrus Prefrontal cortex Primary auditory cortex Primary motor cortex Primary somatosensory cortex Primary visual cortex Pulvinar nucleus Putamen Secondary visual cortex Septal nuclear complex Subiculum Substantia innominata

42-year-old stage 29-year-old stage 58-year-old stage

Normal

Male

Snmc-seq2

Nucleus

Homo sapiens

Cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Vascular leptomeningeal cell Macroglial cell Astrocyte

Brodmann (1909) area 19 Brodmann (1909) area 24 Brodmann (1909) area 25 Brodmann (1909) area 38 Brodmann (1909) area 46 Agranular insular cortex Amygdala Anterior cingulate cortex Basolateral amygdaloid nuclear complex Bed nucleus of stria terminalis Body of caudate nucleus Central amygdaloid nucleus Central nucleus of inferior colliculus Cerebellum Claustrum of brain Corticomedial nuclear complex Cuneus cortex Dentate gyrus of hippocampal formation Globus pallidus Granular insular cortex Hippocampal field Hippocampal formation Inferior temporal gyrus Insula Lateral entorhinal cortex Medial dorsal nucleus of thalamus Medial entorhinal cortex Middle temporal gyrus Nucleus accumbens Parietal cortex Piriform cortex Pontine nuclear group Posterior parahippocampal gyrus Prefrontal cortex Primary auditory cortex Primary motor cortex Primary somatosensory cortex Primary visual cortex Pulvinar nucleus Putamen Secondary visual cortex Septal nuclear complex Subiculum Substantia innominata

42-year-old stage 29-year-old stage 58-year-old stage

Normal

Male

Snmc-seq2

Nucleus

Homo sapiens

Neutrophil Podocyte Renal principal cell Stromal cell Natural killer cell Renal beta-intercalated cell Kidney tubule cell Glomerular endothelial cell Tissue-resident macrophage Endothelial cell Mast cell Cd8-positive, alpha-beta t cell Kidney resident macrophage Peritubular capillary endothelial cell Erythroid lineage cell Classical monocyte Epithelial cell of proximal tubule Epithelial cell Plasmacytoid dendritic cell Renal intercalated cell Renal alpha-intercalated cell Macrophage Kidney pelvis urothelial cell Kidney connecting tubule epithelial cell Innate lymphoid cell Kidney loop of henle thick ascending limb epithelial cell Vasa recta cell Embryonic fibroblast Kidney loop of henle epithelial cell Obsolete somatic cell Cd4-positive, alpha-beta t cell Mesenchymal cell Type i nk t cell Megakaryocyte B cell Neuron Mesenchymal stem cell Monocyte Myofibroblast cell Epithelial fate stem cell Conventional dendritic cell Fibroblast Dendritic cell Non-classical monocyte

Metanephros Adult mammalian kidney

13th week post-fertilization stage 53-year-old stage 14th week post-fertilization stage 17th week post-fertilization stage 72-year-old stage Carnegie stage 23 10th week post-fertilization stage Carnegie stage 22

Normal

Female Male

10x genomics v2

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Normal

Male

Slide-seqv2

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell B cell Pericyte Cerebral cortex neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Putamen

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Retinal ganglion cell Rod bipolar cell Cone retinal bipolar cell Retinal astrocyte Microglial cell Retinal cone cell Retinal rod cell Retina horizontal cell Amacrine cell Pericyte Mueller cell Retinal blood vessel endothelial cell

Fovea centralis Perifoveal part of retina

71-year-old stage 59-year-old stage 78-year-old stage 63-year-old stage

Macular degeneration Normal

Female Male

10x 3' v3

Cell

Homo sapiens

T cell Neutrophil Muscle cell Stromal cell Natural killer cell Erythrocyte Conjunctival epithelial cell Endothelial cell Mature nk t cell Retinal ganglion cell Retinal pigment epithelial cell Mast cell Corneal epithelial cell Cd8-positive, alpha-beta t cell Erythroid lineage cell Schwann cell Classical monocyte Smooth muscle cell Intermediate monocyte Myeloid dendritic cell Adipocyte Retinal bipolar neuron Macrophage Microglial cell Radial glial cell Plasma cell Cd4-positive, alpha-beta t cell Retina horizontal cell Acinar cell B cell Mueller cell Myeloid leukocyte Monocyte Eye photoreceptor cell Basophil Retinal blood vessel endothelial cell Fibroblast Keratocyte Non-classical monocyte

Anterior segment of eyeball Chorioretinal region Conjunctiva Cornea Eye Eyelid Lacrimal gland Ocular surface region Posterior segment of eyeball Retinal neural layer Sclera

57-year-old stage 45-year-old stage 46-year-old stage 40-year-old stage

Normal

Female Male

Smart-seq2 10x 3' v3

Cell

Homo sapiens

T cell Neutrophil Mast cell Immature nk t cell Macrophage B cell Monocyte Dendritic cell

Omental fat pad Subcutaneous adipose tissue

71-year-old stage 53-year-old stage 54-year-old stage 68-year-old stage 29-year-old stage 51-year-old stage 57-year-old stage 49-year-old stage 24-year-old stage 35-year-old stage 58-year-old stage 59-year-old stage 41-year-old stage 36-year-old stage 73-year-old stage

Normal

Female Male

10x 3' v3 Drop-seq

Nucleus Cell