Homo sapiens

Cd8-positive, alpha-beta t cell Exhausted t cell Cd4-positive helper t cell Regulatory t cell Myeloid cell Plasma cell Malignant cell B cell

Lung

61-year-old stage

Renal cell carcinoma

Male

Slide-seqv2

Homo sapiens

Natural killer cell Gamma-delta t cell Lung resident memory cd8-positive, alpha-beta t cell Effector cd4-positive, alpha-beta t cell Lung resident memory cd4-positive, alpha-beta t cell Innate lymphoid cell Regulatory t cell Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Effector memory cd8-positive, alpha-beta t cell

Bronchus Lung Trachea Upper lobe of left lung Lower lobe of left lung

66-year-old stage 75-year-old stage Sixth decade stage 29-year-old stage 35-year-old stage Seventh decade stage 58-year-old stage 59-year-old stage 64-year-old stage

Normal

Female Male

10x 5' transcription profiling 10x 3' v3 10x 3' v2

Cell Nucleus

Homo sapiens

Corneal epithelial cell Basal cell Leukocyte Transit amplifying cell Fibroblast Corneal endothelial cell

Cornea

66-year-old stage 65-year-old stage 30-year-old stage 33-year-old stage 47-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Malignant cell Mesothelial cell

Pleural effusion

Adult stage

Triple-negative breast carcinoma Luminal b breast carcinoma

10x 3' v3 10x 3' v2

Cell

Danio rerio

Retinal ganglion cell Retinal pigment epithelial cell Retinal bipolar neuron Retina horizontal cell Amacrine cell Retinal progenitor cell Eye photoreceptor cell Purkinje cell

Inner nuclear layer of retina Outer nuclear layer of retina Ganglionic layer of retina Cerebellum Pigmented layer of retina Retina

Pharyngula:prim-15 Hatching:long-pec Larval:protruding-mouth Pharyngula:prim-25 Segmentation:26+ somites Segmentation:14-19 somites Hatching:pec-fin Segmentation:20-25 somites Pharyngula:high-pec Pharyngula:prim-5

Normal

Sci-rna-seq3

Nucleus

Callithrix jacchus

Endothelial cell

Brodmann (1909) area 25 Lateral amygdaloid nucleus Dorsolateral prefrontal cortex Thalamic complex Parietal cortex Medial dorsal nucleus of thalamus Primary visual cortex Ventral lateral nucleus of thalamus Brainstem Temporal cortex Lateral nuclear group of thalamus Caudate nucleus Prefrontal cortex Anterior hypothalamic region Temporal lobe Central amygdaloid nucleus Nucleus accumbens Ventral thalamus Basal forebrain Primary somatosensory cortex Primary auditory cortex Brodmann (1909) area 23 Primary motor cortex Dorsal thalamus Hippocampal formation Secondary visual cortex Posterior hypothalamic region Lateral geniculate body Putamen Medial amygdaloid nucleus Amygdala

Late adult stage Prime adult stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Cd8-positive, alpha-beta t cell Exhausted t cell Cd4-positive helper t cell Regulatory t cell Myeloid cell Plasma cell Malignant cell T follicular helper cell B cell

Lymph node

Adult stage

Metastatic melanoma

Slide-seqv2

Drosophila melanogaster

Hemocyte

Antenna

Day 5 of adulthood

Normal

Mixed sex Female Male

10x 3' v3

Mus musculus

Splanchnic mesodermal cell Cardiac muscle cell

Embryo

Theiler stage 22 Theiler stage 27 Theiler stage 24 Theiler stage 18 Theiler stage 25 Theiler stage 14 Theiler stage 16 Theiler stage 17 Theiler stage 15 Theiler stage 19 Theiler stage 26 Theiler stage 13 Theiler stage 20 Theiler stage 12 Theiler stage 21 Theiler stage 23

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Cd8-positive, alpha-beta t cell Exhausted t cell Cd4-positive helper t cell Regulatory t cell Myeloid cell Plasma cell Malignant cell T follicular helper cell B cell

Lymph node

Adult stage

Metastatic melanoma

Slide-seqv2

Homo sapiens

L2/3 intratelencephalic projecting glutamatergic neuron L4/5 intratelencephalic projecting glutamatergic neuron Oligodendrocyte Lamp5 gabaergic interneuron L3 intratelencephalic projecting glutamatergic neuron Microglial cell Vip gabaergic interneuron L5 extratelencephalic projecting glutamatergic cortical neuron L6b glutamatergic cortical neuron L6 corticothalamic-projecting glutamatergic cortical neuron Pvalb gabaergic interneuron Near-projecting glutamatergic cortical neuron L6 intratelencephalic projecting glutamatergic neuron Astrocyte Sst gabaergic interneuron

Brodmann (1909) area 46

71-year-old stage 30-year-old stage 74-year-old stage 23-year-old stage 25-year-old stage 70-year-old stage 73-year-old stage

Normal

Female Male

Mct-seq

Nucleus

Homo sapiens

Central memory cd4-positive, alpha-beta t cell T cell Effector cd8-positive, alpha-beta t cell Gamma-delta t cell Cd8-positive, alpha-beta cytotoxic t cell Naive thymus-derived cd4-positive, alpha-beta t cell Effector memory cd8-positive, alpha-beta t cell

T cell Blood

Adult stage

B-cell acute lymphoblastic leukemia

Female Male

10x 5' v1

Cell