Homo sapiens

Endothelial cell of lymphatic vessel Endothelial cell Smooth muscle cell Pericyte

Subcutaneous adipose tissue Omental fat pad

71-year-old stage 53-year-old stage 68-year-old stage 29-year-old stage 51-year-old stage 57-year-old stage 49-year-old stage 24-year-old stage 35-year-old stage 58-year-old stage 59-year-old stage 41-year-old stage 36-year-old stage 73-year-old stage

Normal

Female Male

10x 3' v3 Drop-seq

Nucleus Cell

Homo sapiens

T cell Neutrophil Granulocyte Natural killer cell Multiciliated columnar cell of tracheobronchial tree Endothelial cell Mature nk t cell Mast cell Cd8-positive, alpha-beta t cell Basal cell Ionocyte Smooth muscle cell Intermediate monocyte Connective tissue cell Plasmacytoid dendritic cell Myeloid dendritic cell Macrophage Innate lymphoid cell Regulatory t cell Plasma cell Cd4-positive, alpha-beta t cell Tracheal goblet cell B cell Serous cell of epithelium of trachea Monocyte Mucus secreting cell Fibroblast Non-classical monocyte

Trachea

61-year-old stage 56-year-old stage 59-year-old stage 67-year-old stage 45-year-old stage 60-year-old stage

Normal

Female Male

Smart-seq2 10x 3' v3

Cell

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2

Homo sapiens

Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell

Renal medulla

56-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Activated cd4-positive, alpha-beta t cell, human Dendritic cell, human Intestine goblet cell Endothelial cell of lymphatic vessel Gamma-delta t cell Mast cell Cd8-positive, alpha-beta t cell Iga plasma cell Endothelial cell of artery Intestinal tuft cell Plasmacytoid dendritic cell Igg plasma cell Enteroendocrine cell Intestinal crypt stem cell Memory b cell Regulatory t cell Myeloid cell Glial cell Enterocyte Plasma cell Cd4-positive, alpha-beta t cell Vein endothelial cell Transit amplifying cell T follicular helper cell B cell Pericyte Monocyte Myofibroblast cell Conventional dendritic cell Fibroblast M cell of gut

Ileum

13-year-old stage 12-year-old stage 9-year-old stage 14-year-old stage 11-year-old stage 6-year-old stage 10-year-old stage 4-year-old stage

Normal Crohn disease

Female Male

10x 3' v2

Cell

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Normal

Male

Slide-seqv2

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Autosomal dominant polycystic kidney disease

Male

Slide-seqv2

Homo sapiens

T cell Lymphocyte of b lineage Natural killer cell Macrophage Myeloid cell Dendritic cell

Pleural effusion

Adult stage

Triple-negative breast carcinoma Luminal b breast carcinoma Luminal a breast carcinoma

10x 3' v3

Cell

Mus musculus

Glutamatergic neuron Endothelial cell Oligodendrocyte Smooth muscle cell Macrophage Leptomeningeal cell Pericyte Astrocyte Gabaergic neuron

Primary visual cortex Frontal cortex

9-week-old stage 12-week-old stage 11-week-old stage 7-week-old stage 10-week-old stage 8-week-old stage

Normal

Female Male

Smart-seq v4

Cell

Homo sapiens

Glioblast Glutamatergic neuron Erythrocyte Oligodendrocyte precursor cell Cajal-retzius cell Microglial cell Forebrain neuroblast Radial glial cell Progenitor cell Gabaergic neuron

Telencephalon

10th week post-fertilization stage

Normal

10x 3' v3

Cell

Mus musculus

Oligodendrocyte precursor cell Oligodendrocyte Dopaminergic neuron Microglial cell Brain microvascular endothelial cell Astrocyte

Normal

10x 3' v2

Homo sapiens

Subcutaneous adipocyte Mesenchymal stem cell of adipose tissue

Subcutaneous adipose tissue

34-year-old stage 24-year-old stage 33-year-old stage 41-year-old stage

Normal

Female Male

10x 3' v3

Nucleus