Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Primary somatosensory cortex

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Podocyte Parietal epithelial cell Endothelial cell Kidney loop of henle thin descending limb epithelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney connecting tubule epithelial cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Pericyte Kidney proximal convoluted tubule epithelial cell

Cortex of kidney

62-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Dentate gyrus granule cell Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron Hippocampal ca4 neuron

Hippocampal field

42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Endothelial cell of lymphatic vessel Endothelial cell Mast cell Macrophage Mural cell Subcutaneous adipocyte Mesenchymal stem cell of adipose tissue B cell Dendritic cell

Subcutaneous adipose tissue

34-year-old stage 24-year-old stage 33-year-old stage 41-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Iga plasma cell Igg plasma cell

Colorectum

69-year-old stage 91-year-old stage 42-year-old stage 89-year-old stage 71-year-old stage 53-year-old stage 61-year-old stage 52-year-old stage 48-year-old stage 56-year-old stage 55-year-old stage 82-year-old stage 50-year-old stage 90-year-old stage 51-year-old stage 57-year-old stage 72-year-old stage 81-year-old stage 49-year-old stage 35-year-old stage 58-year-old stage 59-year-old stage 67-year-old stage 77-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 70-year-old stage 60-year-old stage 78-year-old stage 36-year-old stage 47-year-old stage 76-year-old stage 73-year-old stage

Normal Colon adenocarcinoma

Female Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

T cell Central nervous system macrophage Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell B cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Brodmann (1909) area 25

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Mononuclear leukocyte Podocyte Renal principal cell Parietal epithelial cell Renal beta-intercalated cell Endothelial cell Epithelial cell of proximal tubule Renal alpha-intercalated cell Kidney distal convoluted tubule epithelial cell Kidney loop of henle thick ascending limb epithelial cell Kidney proximal straight tubule epithelial cell B cell Kidney loop of henle thin ascending limb epithelial cell Kidney proximal convoluted tubule epithelial cell Fibroblast

Cortex of kidney

53-year-old stage 61-year-old stage 52-year-old stage 51-year-old stage 57-year-old stage 74-year-old stage 59-year-old stage 78-year-old stage

Normal Type 2 diabetes mellitus

Female Male

10x scatac-seq

Nucleus

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2

Homo sapiens

T cell Neutrophil Podocyte Renal principal cell Endothelial cell of lymphatic vessel Parietal epithelial cell Kidney interstitial alternatively activated macrophage Renal beta-intercalated cell Renal interstitial pericyte Epithelial cell of early distal convoluted tubule Endothelial cell of arteriole Peritubular capillary endothelial cell Kidney outer medulla collecting duct intercalated cell Epithelial cell of proximal tubule Plasmacytoid dendritic cell Vasa recta descending limb cell Myeloid dendritic cell Lymphocyte Kidney connecting tubule epithelial cell Kidney connecting tubule principal cell Kidney loop of henle thick ascending limb epithelial cell Vasa recta ascending limb cell Epithelial cell of proximal tubule segment 3 Macula densa epithelial cell Plasma cell Mesangial cell Kidney collecting duct alpha-intercalated cell B cell Vascular associated smooth muscle cell Glomerular capillary endothelial cell Kidney proximal convoluted tubule epithelial cell Fibroblast Non-classical monocyte

Cortex of kidney

69-year-old stage 53-year-old stage 68-year-old stage 50-year-old stage 57-year-old stage 72-year-old stage 74-year-old stage 59-year-old stage 67-year-old stage

Normal

Female Male

10x multiome

Nucleus

Homo sapiens

Trophoblast giant cell Extravillous trophoblast Placental villous trophoblast Syncytiotrophoblast cell

Decidua basalis

9th week post-fertilization stage

Normal

Female

10x multiome 10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Brodmann (1909) area 38

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Cd8-positive, alpha-beta t cell Exhausted t cell Cd4-positive helper t cell Regulatory t cell Myeloid cell Plasma cell Malignant cell B cell

Lung

61-year-old stage

Renal cell carcinoma

Male

Slide-seqv2