Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Sst chodl gabaergic interneuron Astrocyte Fibroblast

Brodmann (1909) area 43

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Efferent neuron Olfactory receptor cell

Embryo

Theiler stage 22 Theiler stage 27 Theiler stage 24 Theiler stage 18 Theiler stage 25 Theiler stage 14 Theiler stage 16 Theiler stage 17 Theiler stage 15 Theiler stage 19 Theiler stage 26 Theiler stage 20 Theiler stage 21 Theiler stage 23

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Fast muscle cell Neutrophil Natural killer cell Endothelial cell of lymphatic vessel Erythrocyte Thymic fibroblast type 1 Endothelial cell Hematopoietic precursor cell Mast cell Cd8-positive, alpha-beta t cell Myo-medullary thymic epithelial cell Endothelial cell of artery Capillary endothelial cell Smooth muscle cell Thymic fibroblast type 2 Naive thymus-derived cd8-positive, alpha-beta t cell Thymocyte Macrophage Innate lymphoid cell Neuro-medullary thymic epithelial cell Plasma cell Cd4-positive, alpha-beta t cell Vein endothelial cell Cd8-positive, alpha-beta thymocyte Cd4-positive, alpha-beta thymocyte T follicular helper cell Medullary thymic epithelial cell Mesothelial cell B cell Naive thymus-derived cd4-positive, alpha-beta t cell Monocyte Vascular associated smooth muscle cell Fibroblast

Thymus

61-year-old stage 56-year-old stage 59-year-old stage 45-year-old stage 60-year-old stage

Normal

Female Male

10x 5' v2 Smart-seq2 10x 3' v3

Cell

Danio rerio

Muscle cell Adaxial cell Endothelial cell Tendon cell Muscle precursor cell Mesodermal cell Mesenchymal cell Myoblast

Craniocervical muscle Presomitic mesoderm Somite Multicellular anatomical structure Pharyngeal arch Myotome Hypaxial musculature

Pharyngula:prim-15 Hatching:long-pec Larval:protruding-mouth Pharyngula:prim-25 Segmentation:26+ somites Segmentation:14-19 somites Hatching:pec-fin Segmentation:20-25 somites Pharyngula:high-pec Pharyngula:prim-5

Normal

Sci-rna-seq3

Nucleus

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Thalamic excitatory neuron B cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Central amygdaloid nucleus

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Neutrophil Natural killer cell Endothelial cell of lymphatic vessel Erythrocyte Endothelial cell Mature nk t cell Mast cell Cd8-positive, alpha-beta t cell Endothelial cell of artery Capillary endothelial cell Classical monocyte Smooth muscle cell Intermediate monocyte Myeloid dendritic cell Macrophage Plasma cell Cd4-positive, alpha-beta t cell Vein endothelial cell B cell Pericyte Monocyte Basophil Fibroblast Non-classical monocyte

Inferior vena cava Thoracic aorta Aorta Coronary artery Abdominal aorta Left coronary artery Vasculature

61-year-old stage 56-year-old stage 59-year-old stage 45-year-old stage 60-year-old stage

Normal

Female Male

Smart-seq2 10x 3' v3

Cell

Homo sapiens

Granulosa cell Blood vessel endothelial cell Stromal cell Endothelial cell of lymphatic vessel Leukocyte Smooth muscle cell Epithelial cell Theca cell

Ovary

52-year-old stage 54-year-old stage 29-year-old stage 28-year-old stage 51-year-old stage 49-year-old stage 23-year-old stage 27-year-old stage

Normal

Female

10x 3' v3

Nucleus

Homo sapiens

Glutamatergic neuron Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Mature microglial cell Mature astrocyte Gabaergic neuron

Entorhinal cortex

87-year-old stage 71-year-old stage 82-year-old stage 50-year-old stage 72-year-old stage 80 year-old and over stage 77-year-old stage 60-year-old stage

Normal Alzheimer disease

Male

10x 3' v2

Nucleus

Homo sapiens

Microglial cell

Dorsolateral prefrontal cortex

69-year-old stage 75-year-old stage 42-year-old stage 89-year-old stage 87-year-old stage 85-year-old stage 88-year-old stage 68-year-old stage 29-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 80-year-old stage 72-year-old stage 81-year-old stage 80 year-old and over stage 77-year-old stage 86-year-old stage 70-year-old stage 78-year-old stage 83-year-old stage

Normal Dementia

Female Male

10x multiome 10x 3' v3

Nucleus

Homo sapiens

Luminal cell of prostate epithelium Secretory cell Epithelial cell of urethra Basal cell of prostate epithelium

Transition zone of prostate Peripheral zone of prostate

71-year-old stage 29-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 67-year-old stage 31-year-old stage 73-year-old stage

Benign prostatic hyperplasia Normal

Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Granulocyte Mature conventional dendritic cell Plasmacytoid dendritic cell Macrophage Monocyte Conventional dendritic cell Dendritic cell

Colorectum

69-year-old stage 91-year-old stage 42-year-old stage 89-year-old stage 71-year-old stage 53-year-old stage 61-year-old stage 52-year-old stage 48-year-old stage 56-year-old stage 55-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 90-year-old stage 51-year-old stage 57-year-old stage 72-year-old stage 81-year-old stage 49-year-old stage 35-year-old stage 58-year-old stage 59-year-old stage 67-year-old stage 77-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 70-year-old stage 60-year-old stage 78-year-old stage 36-year-old stage 47-year-old stage 76-year-old stage 73-year-old stage

Normal Colon adenocarcinoma

Female Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Neutrophil Transit amplifying cell of small intestine Endothelial cell of venule Natural killer cell Endothelial cell of lymphatic vessel Enterocyte of epithelium proper of small intestine Gamma-delta t cell Activated cd4-positive, alpha-beta t cell Best4+ enterocyte Mast cell Cd8-positive, alpha-beta t cell Endothelial cell of arteriole Enteroglial cell Intestinal tuft cell Myeloid dendritic cell Enterocyte of epithelium proper of jejunum Enterocyte of epithelium proper of duodenum Small intestine goblet cell Macrophage Innate lymphoid cell Regulatory t cell Paneth cell of epithelium of small intestine Activated cd8-positive, alpha-beta t cell Enteroendocrine cell of small intestine Enterocyte of epithelium proper of ileum Plasma cell Cd4-positive, alpha-beta t cell B cell Pericyte Naive thymus-derived cd4-positive, alpha-beta t cell Intestinal crypt stem cell of small intestine Monocyte Myofibroblast cell Fibroblast

Duodenum Small intestine Ileum Jejunum

37-year-old stage 61-year-old stage 56-year-old stage 59-year-old stage 60-year-old stage

Normal

Female Male

Smart-seq2 10x 3' v3

Cell