Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell B cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Lateral entorhinal cortex

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2

Homo sapiens

Central nervous system macrophage Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Cerebellar granule cell Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Pericyte Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Body of caudate nucleus

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Cerebellar inhibitory gabaergic interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Extrastriate cortex

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Oligodendrocyte precursor cell Oligodendrocyte Schwann cell

Spinal cord

3-6 month-old mature stage

Spinal cord injury Normal

Female

10x 3' v2

Nucleus

Homo sapiens

Central memory cd4-positive, alpha-beta t cell T cell Natural killer cell Follicular dendritic cell Centrocyte Cd4-positive helper t cell Plasmacytoid dendritic cell Cd8-positive, alpha-beta cytotoxic t cell Centroblast Macrophage Memory b cell Innate lymphoid cell Regulatory t cell Mature b cell Myeloid cell Plasmablast T follicular regulatory cell T follicular helper cell Central memory cd8-positive, alpha-beta t cell Naive b cell Conventional dendritic cell Tonsil germinal center b cell

Tonsil

9-year-old stage 3-year-old stage 5-year-old stage 6-year-old stage

Normal

Female Male

10x 5' v1 10x 3' v2

Cell

Homo sapiens

T cell Nasal mucosa goblet cell Ciliated cell Secretory cell Mast cell Cd8-positive, alpha-beta t cell Inflammatory macrophage Basal cell Ionocyte Plasmacytoid dendritic cell Enteroendocrine cell Cd8-positive, alpha-beta cytotoxic t cell Macrophage Squamous epithelial cell B cell Erythroblast Multiciliated epithelial cell Dendritic cell

Nasopharynx

Sixth decade stage Fifth decade stage Third decade stage Seventh decade stage Fourth decade stage Eighth decade stage Ninth decade stage

Covid-19 Long covid-19 Respiratory failure Normal

Female Male

Seq-well s3

Cell

Homo sapiens

Oligodendrocyte precursor cell

Middle temporal gyrus

69-year-old stage 75-year-old stage 65-year-old stage 89-year-old stage 43-year-old stage 87-year-old stage 85-year-old stage 88-year-old stage 68-year-old stage 29-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 80-year-old stage 72-year-old stage 81-year-old stage 80 year-old and over stage Adult stage 77-year-old stage 86-year-old stage 70-year-old stage 60-year-old stage 78-year-old stage 83-year-old stage

Normal Dementia

Female Male

10x multiome 10x 3' v3

Nucleus

Homo sapiens

Transit amplifying cell of small intestine Endothelial cell of lymphatic vessel Endothelial cell Alpha-beta t cell Mast cell Innate lymphoid cell Paneth cell of epithelium of small intestine Enteroendocrine cell of small intestine Enterocyte of epithelium proper of ileum Glial cell Plasma cell Ileal goblet cell Smooth muscle fiber of ileum B cell Pericyte Progenitor cell Fibroblast Mononuclear phagocyte

Ileum

Prime adult stage

Normal

10x 3' v1 10x 3' v2

Cell

Homo sapiens

Natural killer cell Gamma-delta t cell Mucosal invariant t cell Cd16-negative, cd56-bright natural killer cell, human

Blood

Sixth decade stage Fifth decade stage Seventh decade stage Fourth decade stage

Covid-19 Normal

Female Male

10x 5' v2 10x 5' v1

Cell

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Cerebellar granule cell Medial ganglionic eminence derived interneuron Cerebellar inhibitory gabaergic interneuron Ependymal cell Microglial cell Thalamic excitatory neuron Choroid plexus epithelial cell Bergmann glial cell B cell Pericyte Cerebral cortex neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Purkinje cell Vascular associated smooth muscle cell Astrocyte Unipolar brush cell Fibroblast

Midbrain tectum

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Brodmann (1909) area 24

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus