Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Cerebellar inhibitory gabaergic interneuron Ependymal cell Microglial cell Thalamic excitatory neuron Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron Hippocampal ca4 neuron

Bed nucleus of stria terminalis

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Brodmann (1909) area 13

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Normal

Male

Slide-seqv2

Homo sapiens

T cell Nasal mucosa goblet cell Club cell Keratinizing barrier epithelial cell Ionocyte Respiratory tract epithelial cell Respiratory basal cell Keratinocyte Glandular secretory epithelial cell Professional antigen presenting cell Respiratory tract multiciliated cell

Nasal cavity epithelium

66-year-old stage 34-year-old stage 65-year-old stage 30-year-old stage 59-year-old stage 64-year-old stage 63-year-old stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

T cell Natural killer cell Activated cd4-positive, alpha-beta t cell Mast cell Cd8-alpha-beta-positive, alpha-beta intraepithelial t cell Intestinal tuft cell Enteroendocrine cell Goblet cell Transit amplifying cell of colon Macrophage Intestinal crypt stem cell Follicular b cell Innate lymphoid cell Regulatory t cell Enterocyte Plasma cell Cd4-positive, alpha-beta t cell Cd8-positive, alpha-beta thymocyte Cd4-positive, alpha-beta memory t cell B cell Fibroblast Dendritic cell M cell of gut

Transverse colon epithelium Colonic epithelium Caecum epithelium

Adult stage

Normal

Female Male

10x 3' v1 10x 3' v2

Cell

Homo sapiens

T cell Natural killer cell Retinal ganglion cell Retinal pigment epithelial cell Mast cell Off-bipolar cell Melanocyte On-bipolar cell Rod bipolar cell Endothelial cell of vascular tree Microglial cell Retinal cone cell Retinal rod cell Retina horizontal cell Amacrine cell Pericyte Mueller cell Monocyte Astrocyte Fibroblast

Pigment epithelium of eye Retina

Adult stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Vascular leptomeningeal cell Macroglial cell Astrocyte

Brodmann (1909) area 25 Corticomedial nuclear complex Insula Parietal cortex Brodmann (1909) area 19 Medial dorsal nucleus of thalamus Primary visual cortex Claustrum of brain Inferior temporal gyrus Subiculum Pontine nuclear group Globus pallidus Piriform cortex Hippocampal field Prefrontal cortex Middle temporal gyrus Central amygdaloid nucleus Septal nuclear complex Brodmann (1909) area 38 Lateral entorhinal cortex Medial entorhinal cortex Central nucleus of inferior colliculus Agranular insular cortex Nucleus accumbens Substantia innominata Primary somatosensory cortex Cerebellum Pulvinar nucleus Primary auditory cortex Primary motor cortex Brodmann (1909) area 24 Brodmann (1909) area 46 Posterior parahippocampal gyrus Basolateral amygdaloid nuclear complex Hippocampal formation Granular insular cortex Anterior cingulate cortex Cuneus cortex Dentate gyrus of hippocampal formation Body of caudate nucleus Secondary visual cortex Bed nucleus of stria terminalis Putamen Amygdala

42-year-old stage 29-year-old stage 58-year-old stage

Normal

Male

Snmc-seq2

Nucleus

Homo sapiens

Cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Vascular leptomeningeal cell Macroglial cell Astrocyte

Brodmann (1909) area 25 Corticomedial nuclear complex Insula Parietal cortex Brodmann (1909) area 19 Medial dorsal nucleus of thalamus Primary visual cortex Claustrum of brain Inferior temporal gyrus Subiculum Pontine nuclear group Globus pallidus Piriform cortex Hippocampal field Prefrontal cortex Middle temporal gyrus Central amygdaloid nucleus Septal nuclear complex Brodmann (1909) area 38 Lateral entorhinal cortex Medial entorhinal cortex Central nucleus of inferior colliculus Agranular insular cortex Nucleus accumbens Substantia innominata Primary somatosensory cortex Cerebellum Pulvinar nucleus Primary auditory cortex Primary motor cortex Brodmann (1909) area 24 Brodmann (1909) area 46 Posterior parahippocampal gyrus Basolateral amygdaloid nuclear complex Hippocampal formation Granular insular cortex Anterior cingulate cortex Cuneus cortex Dentate gyrus of hippocampal formation Body of caudate nucleus Secondary visual cortex Bed nucleus of stria terminalis Putamen Amygdala

42-year-old stage 29-year-old stage 58-year-old stage

Normal

Male

Snmc-seq2

Nucleus

Homo sapiens

Neutrophil Podocyte Renal principal cell Stromal cell Natural killer cell Renal beta-intercalated cell Kidney tubule cell Glomerular endothelial cell Tissue-resident macrophage Endothelial cell Mast cell Cd8-positive, alpha-beta t cell Kidney resident macrophage Peritubular capillary endothelial cell Erythroid lineage cell Classical monocyte Epithelial cell of proximal tubule Epithelial cell Plasmacytoid dendritic cell Renal intercalated cell Renal alpha-intercalated cell Macrophage Kidney pelvis urothelial cell Kidney connecting tubule epithelial cell Innate lymphoid cell Kidney loop of henle thick ascending limb epithelial cell Vasa recta cell Embryonic fibroblast Kidney loop of henle epithelial cell Obsolete somatic cell Cd4-positive, alpha-beta t cell Mesenchymal cell Type i nk t cell Megakaryocyte B cell Neuron Mesenchymal stem cell Monocyte Myofibroblast cell Epithelial fate stem cell Conventional dendritic cell Fibroblast Dendritic cell Non-classical monocyte

Adult mammalian kidney Metanephros

13th week post-fertilization stage 53-year-old stage 14th week post-fertilization stage 17th week post-fertilization stage 72-year-old stage Carnegie stage 23 10th week post-fertilization stage Carnegie stage 22

Normal

Female Male

10x genomics v2

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Normal

Male

Slide-seqv2

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell B cell Pericyte Cerebral cortex neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Putamen

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Normal

Male

Slide-seqv2