Mus musculus

Neutrophil Cell Natural killer cell Gamma-delta t cell Mast cell Cd8-positive, alpha-beta t cell Plasmacytoid dendritic cell Macrophage Regulatory t cell Monocyte-derived dendritic cell Plasma cell Cd4-positive, alpha-beta t cell Type i nk t cell Eosinophil B cell Monocyte Conventional dendritic cell Osteoclast Dendritic cell

Cutaneous melanoma

10x 5' transcription profiling

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Cerebellar granule cell Lamp5 lhx6 neuron Medial ganglionic eminence derived interneuron Cerebellar inhibitory gabaergic interneuron Microglial cell B cell Pericyte Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Purkinje cell Vascular associated smooth muscle cell Astrocyte Unipolar brush cell Fibroblast

Pontine nuclear group

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Ependymal cell Astrocyte

Substantia nigra pars compacta

75-year-old stage 91-year-old stage 89-year-old stage 92-year-old stage 82-year-old stage 90-year-old stage 74-year-old stage 49-year-old stage 70-year-old stage 78-year-old stage 76-year-old stage 79-year-old stage 83-year-old stage

Parkinson disease Normal Lewy body dementia

Female Male

10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Primary visual cortex

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Rattus norvegicus

T cell Neutrophil Club cell Ciliated cell Endothelial cell of lymphatic vessel Mast cell Inflammatory macrophage Classical monocyte Smooth muscle cell Plasmacytoid dendritic cell Lung macrophage Cd8-positive, alpha-beta cytotoxic t cell Endothelial cell of umbilical vein Regulatory t cell Fibroblast of lung Neoplastic cell Alveolar macrophage Type ii nk t cell Type i nk t cell Mesothelial cell B cell Naive t cell Conventional dendritic cell Pulmonary alveolar type 2 cell Non-classical monocyte

Pulmonary hypertension owing to lung disease and/or hypoxia Pulmonary hypertension, primary, 1 Normal

Drop-seq

Homo sapiens

T cell Blood vessel endothelial cell Blood vessel smooth muscle cell Macrophage Malignant cell Fibroblast

Axilla

27-year-old stage

Breast cancer

Female

Slide-seqv2

Homo sapiens

Pyramidal neuron Cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Inhibitory interneuron Microglial cell Astrocyte

Tuberous sclerosis Normal Hemimegalencephaly Isolated focal cortical dysplasia

10x 3' v3

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Normal

Male

Slide-seqv2

Homo sapiens

T cell Neutrophil Natural killer cell Endothelial cell of lymphatic vessel Tissue-resident macrophage Mature nk t cell Interstitial cell of cajal Mast cell Cd8-positive, alpha-beta t cell Smooth muscle cell Epithelial cell Enteroendocrine cell Macrophage Regulatory t cell Plasma cell Cd4-positive, alpha-beta t cell B cell Monocyte Fibroblast Mononuclear phagocyte

Stomach Mucosa of stomach Stomach smooth muscle Muscularis mucosae of stomach

56-year-old stage 60-year-old stage

Normal

Female Male

Smart-seq2 10x 3' v3

Cell

Homo sapiens

Macrophage Microglial cell

Substantia nigra pars compacta

75-year-old stage 91-year-old stage 89-year-old stage 92-year-old stage 82-year-old stage 90-year-old stage 74-year-old stage 49-year-old stage 70-year-old stage 78-year-old stage 76-year-old stage 79-year-old stage 83-year-old stage

Parkinson disease Normal Lewy body dementia

Female Male

10x 3' v3

Nucleus

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Normal

Male

Slide-seqv2

Mus musculus

Tracheal epithelial cell

Tracheal epithelial cell

Mature stage

Normal

10x 3' v3

Cell