Homo sapiens

Mesothelial cell

Omental fat pad

53-year-old stage 68-year-old stage 29-year-old stage 24-year-old stage 35-year-old stage 41-year-old stage 36-year-old stage 73-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Mus musculus

Oligodendrocyte Brain microvascular endothelial cell Obsolete excitatory neuron Astrocyte

Normal

10x 3' v2

Mus musculus

Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Normal

Male

Slide-seqv2

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Normal

Male

Slide-seqv2

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Nucleus accumbens

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Valve interstitial cell Endothelial cell Neural cell Cardiac mesenchymal cell Cardiac muscle cell Hematopoietic cell

Outflow tract Outflow tract myocardium

13th week post-fertilization stage Adult stage Carnegie stage 17

Normal

Female Male

10x 3' v3 10x 3' v2

Nucleus

Homo sapiens

Neutrophil Intestinal crypt stem cell of colon Natural killer cell Gamma-delta t cell Enterochromaffin-like cell Best4+ enterocyte Mature nk t cell Colon endothelial cell Mast cell Cd8-positive, alpha-beta t cell Colon macrophage Enteroglial cell Myeloid dendritic cell Transit amplifying cell of colon Tuft cell of colon Paneth cell of colon Innate lymphoid cell Regulatory t cell Plasma cell Cd4-positive, alpha-beta t cell B cell Naive thymus-derived cd4-positive, alpha-beta t cell Myeloid leukocyte Monocyte Large intestine goblet cell Enterocyte of epithelium of large intestine Myofibroblast cell Fibroblast Type l enteroendocrine cell

Ascending colon Large intestine Sigmoid colon

37-year-old stage 61-year-old stage 56-year-old stage 59-year-old stage 60-year-old stage

Normal

Female Male

Smart-seq2 10x 3' v3

Cell

Homo sapiens

Amygdala excitatory neuron L2/3-6 intratelencephalic projecting glutamatergic neuron L5 extratelencephalic projecting glutamatergic cortical neuron L6b glutamatergic cortical neuron L6 corticothalamic-projecting glutamatergic cortical neuron Near-projecting glutamatergic cortical neuron

Corticomedial nuclear complex Primary visual cortex Piriform cortex Middle temporal gyrus Septal nuclear complex Lateral entorhinal cortex Agranular insular cortex Nucleus accumbens Substantia innominata Primary motor cortex Brodmann (1909) area 24 Brodmann (1909) area 46 Bed nucleus of stria terminalis Putamen Amygdala

42-year-old stage 29-year-old stage 58-year-old stage

Normal

Male

Snm3c-seq

Nucleus

Homo sapiens

Amygdala excitatory neuron L2/3-6 intratelencephalic projecting glutamatergic neuron L5 extratelencephalic projecting glutamatergic cortical neuron L6b glutamatergic cortical neuron L6 corticothalamic-projecting glutamatergic cortical neuron Near-projecting glutamatergic cortical neuron

Corticomedial nuclear complex Primary visual cortex Piriform cortex Middle temporal gyrus Septal nuclear complex Lateral entorhinal cortex Agranular insular cortex Nucleus accumbens Substantia innominata Primary motor cortex Brodmann (1909) area 24 Brodmann (1909) area 46 Bed nucleus of stria terminalis Putamen Amygdala

42-year-old stage 29-year-old stage 58-year-old stage

Normal

Male

Snm3c-seq

Nucleus

Homo sapiens

Direct pathway medium spiny neuron Pvalb chandelier gabaergic interneuron Lamp5 gabaergic interneuron Vip gabaergic interneuron Indirect pathway medium spiny neuron Pvalb gabaergic interneuron Central nervous system neuron Sncg gabaergic interneuron Sst gabaergic interneuron

Corticomedial nuclear complex Primary visual cortex Globus pallidus Piriform cortex Middle temporal gyrus Septal nuclear complex Lateral entorhinal cortex Agranular insular cortex Nucleus accumbens Substantia innominata Primary motor cortex Brodmann (1909) area 24 Brodmann (1909) area 46 Body of caudate nucleus Bed nucleus of stria terminalis Putamen Amygdala

42-year-old stage 29-year-old stage 58-year-old stage

Normal

Male

Snm3c-seq

Nucleus

Homo sapiens

Direct pathway medium spiny neuron Pvalb chandelier gabaergic interneuron Lamp5 gabaergic interneuron Vip gabaergic interneuron Indirect pathway medium spiny neuron Pvalb gabaergic interneuron Central nervous system neuron Sncg gabaergic interneuron Sst gabaergic interneuron

Corticomedial nuclear complex Primary visual cortex Globus pallidus Piriform cortex Middle temporal gyrus Septal nuclear complex Lateral entorhinal cortex Agranular insular cortex Nucleus accumbens Substantia innominata Primary motor cortex Brodmann (1909) area 24 Brodmann (1909) area 46 Body of caudate nucleus Bed nucleus of stria terminalis Putamen Amygdala

42-year-old stage 29-year-old stage 58-year-old stage

Normal

Male

Snm3c-seq

Nucleus

Drosophila melanogaster

Neuron Fat cell Tracheolar cell Glial cell Somatic precursor cell Male germline cell Hemocyte Eye photoreceptor cell Epithelial cell Muscle cell Adult tracheocyte Adult neuron Sensory neuron Oenocyte Neuron Female germline cell

Trachea

Day 5 of adulthood

Normal

Female Male

10x 3' v3