Homo sapiens

Megakaryocyte Non-classical monocyte Natural killer cell Endothelial cell of arteriole Schwann cell Classical monocyte Mast cell Vascular associated smooth muscle cell Fibroblast Helper t cell Pericyte Myelinating schwann cell Macrophage Dendritic cell T cell Non-myelinating schwann cell Retinal pigment epithelial cell Cytotoxic t cell Endothelial cell of venule Vein endothelial cell Plasma cell Plasmacytoid dendritic cell Innate lymphoid cell Smooth muscle cell Fenestrated endothelial cell Endothelial cell of artery B cell Melanocyte

Chorioretinal region

64-year-old stage 50-year-old stage 65-year-old stage 68-year-old stage 82-year-old stage 43-year-old stage 63-year-old stage 90 year-old and over stage 58-year-old stage 34-year-old stage 86-year-old stage 25-year-old stage 11-year-old stage 21-year-old stage 53-year-old stage 46-year-old stage 77-year-old stage 47-year-old stage 32-year-old stage 81-year-old stage 69-year-old stage Newborn stage (0-28 days) 60-year-old stage 62-year-old stage 16-year-old stage 10-year-old stage 30-year-old stage 72-year-old stage 27-year-old stage 17-year-old stage 71-year-old stage

Normal

Female Male

10x 3' v3 10x 5' v2

Nucleus

Homo sapiens

Neuron

Basal forebrain Brain Caudate-putamen Cerebellar peduncular complex Cerebellum Cerebral subcortex Choroid plexus epithelium Cortex Diencephalon Dorsal plus ventral thalamus Entorhinal cortex Forebrain Frontal cortex Head Hindbrain Hippocampal formation Hypothalamus Lower layers of the cortex Medulla oblongata Metencephalon Midbrain Midbrain tectum Occipital cortex Pons Striatum Telencephalon Upper layers of the cortex

15th week post-fertilization stage 11th week post-fertilization stage Carnegie stage 15 14th week post-fertilization stage Carnegie stage 18 9th week post-fertilization stage 13th week post-fertilization stage Carnegie stage 22 12th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 16 Carnegie stage 20

Normal

10x 3' v2 10x 3' v3

Cell

Homo sapiens

Natural killer cell Cd16-negative, cd56-bright natural killer cell, human Innate lymphoid cell Cd16-positive, cd56-dim natural killer cell, human

Blood

80 year-old and over stage 85-year-old stage 80-year-old stage 64-year-old stage 50-year-old stage 52-year-old stage 65-year-old stage 68-year-old stage 82-year-old stage 43-year-old stage 41-year-old stage 63-year-old stage 58-year-old stage 73-year-old stage 75-year-old stage 84-year-old stage 42-year-old stage 49-year-old stage 86-year-old stage 59-year-old stage 54-year-old stage 66-year-old stage 61-year-old stage 57-year-old stage 74-year-old stage 83-year-old stage 89-year-old stage 53-year-old stage 46-year-old stage 56-year-old stage 55-year-old stage 44-year-old stage 77-year-old stage 78-year-old stage 47-year-old stage 45-year-old stage 70-year-old stage 81-year-old stage 76-year-old stage 69-year-old stage 67-year-old stage 40-year-old stage 88-year-old stage 87-year-old stage 60-year-old stage 62-year-old stage 79-year-old stage 72-year-old stage 48-year-old stage 51-year-old stage 71-year-old stage

Normal Cytomegalovirus infection

Female Male

10x gene expression flex

Cell

Mus musculus

Splanchnic mesodermal cell Midbrain dopaminergic neuron Premigratory neural crest cell Surface ectodermal cell Notochordal cell Neuroplacodal cell Anterior visceral endoderm cell Mesodermal cell Paraxial cell Spinal cord interneuron Cardiac valve cell Gut endothelial cell Endodermal cell Hemangioblast Primitive red blood cell Neurectodermal cell

Embryo

Theiler stage 14

Normal

Slide-seqv2

Mus musculus

Bladder urothelial cell Gingival epithelial cell Epithelial cell of thymus Corneal epithelial cell Auditory epithelial cell Kidney epithelial cell Endo-epithelial cell Intrahepatic cholangiocyte Notochordal cell Ectodermal cell Kidney collecting duct intercalated cell Conjunctiva goblet cell Epithelial cell of proximal tubule Kidney collecting duct principal cell Epithelial cell of thyroid gland Intermediate mesodermal cell Lens epithelial cell Kidney distal convoluted tubule epithelial cell Kidney loop of henle ascending limb epithelial cell Epithelial cell Epithelial cell of amnion Olfactory epithelial cell Ureteric bud cell Pituitary gland cell Kidney connecting tubule epithelial cell Metanephric mesenchyme stem cell Epithelial cell of parathyroid gland Primordial germ cell Pancreatic acinar cell Keratinocyte Podocyte

Embryo

Theiler stage 26 Theiler stage 16 Theiler stage 13 Theiler stage 14 Theiler stage 19 Theiler stage 25 Theiler stage 23 Theiler stage 17 Theiler stage 27 Theiler stage 20 Theiler stage 22 Theiler stage 12 Theiler stage 24 Theiler stage 21 Theiler stage 15 Theiler stage 18

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Radial glial cell

Basal forebrain Brain Caudate-putamen Cerebellar peduncular complex Cerebellum Cerebral subcortex Choroid plexus epithelium Cortex Diencephalon Dorsal plus ventral thalamus Entorhinal cortex Forebrain Frontal cortex Head Hindbrain Hippocampal formation Hypothalamus Lower layers of the cortex Medulla oblongata Metencephalon Midbrain Midbrain tectum Occipital cortex Pons Striatum Telencephalon Upper layers of the cortex

15th week post-fertilization stage 11th week post-fertilization stage Carnegie stage 15 14th week post-fertilization stage Carnegie stage 18 9th week post-fertilization stage 13th week post-fertilization stage Carnegie stage 22 12th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 16 Carnegie stage 20

Normal

10x 3' v2 10x 3' v3

Cell

Homo sapiens

Monocyte Dendritic cell Hematopoietic multipotent progenitor cell Hematopoietic precursor cell Antibody secreting cell Lymphocyte Endothelial cell B cell

Blood

85-year-old stage 80-year-old stage 26-year-old stage 50-year-old stage 65-year-old stage 68-year-old stage 82-year-old stage 43-year-old stage 41-year-old stage 63-year-old stage 37-year-old stage 58-year-old stage 73-year-old stage 75-year-old stage 93-year-old stage 84-year-old stage 34-year-old stage 31-year-old stage 49-year-old stage 86-year-old stage 59-year-old stage 54-year-old stage 25-year-old stage 92-year-old stage 66-year-old stage 61-year-old stage 57-year-old stage 83-year-old stage 89-year-old stage 46-year-old stage 55-year-old stage 44-year-old stage 77-year-old stage 78-year-old stage 47-year-old stage 36-year-old stage 32-year-old stage 70-year-old stage 81-year-old stage 76-year-old stage 69-year-old stage 67-year-old stage 40-year-old stage 90-year-old stage 60-year-old stage 62-year-old stage 39-year-old stage 28-year-old stage 38-year-old stage 23-year-old stage 30-year-old stage 72-year-old stage 48-year-old stage 51-year-old stage 71-year-old stage 33-year-old stage

Normal Myelodysplastic syndrome Hematologic disorder Myelodysplastic/myeloproliferative disease

Female Male

10x 3' v3

Cell

Drosophila melanogaster

Adult renal stem cell Hemocyte Adult alary muscle cell Enteroendocrine cell Ovarian sheath muscle cell Adult brain perineurial glial cell Germline cell Follicle cell Epithelial cell Visceral muscle cell Escort cell Cell body glial cell Subperineurial glial cell Indirect flight muscle cell Embryonic/larval crystal cell Muscle cell Sensory neuron Eo sensory organ accessory cell Mechanosensory neuron Adult heart ventral longitudinal muscle cell Adult somatic muscle cell of head

Heart Trachea Body wall Fat body Gut Adult oenocyte Antenna Haltere Head Leg Ovary Testis Wing Malpighian tubule Body proper Male reproductive gland Insect proboscis

Day 2 of adulthood Day 5 of adulthood Day 3 of adulthood Day 1 of adulthood

Normal

Female Male Mixed sex

10x 3' v3

Rattus norvegicus

Peripheral nervous system neuron Natural killer cell Schwann cell Cd8-positive, alpha-beta t cell Endothelial cell of lymphatic vessel Mast cell Vascular associated smooth muscle cell Fibroblast Cardiac neuron Monocyte Pericyte Eukaryotic cell Club cell White adipocyte Mesothelial cell of epicardium Cardiac atrium fibroblast Cardiac pacemaker cell of sinoatrial node Macrophage Dendritic cell T cell Cd14-low, cd16-positive monocyte Non-myelinating schwann cell Brown adipocyte Cardiac ventricle fibroblast Regular ventricular cardiac myocyte Cycling t cell Regular atrial cardiac myocyte Fibroblast of cardiac tissue Myocyte of sinoatrial node Endothelial cell Cardiac endothelial cell B cell Myocyte of atrioventricular node

Normal

10x 3' v2

Homo sapiens

Monocyte Dendritic cell Hematopoietic multipotent progenitor cell Hematopoietic precursor cell Antibody secreting cell Lymphocyte Endothelial cell B cell

Blood

85-year-old stage 80-year-old stage 64-year-old stage 26-year-old stage 91-year-old stage 50-year-old stage 52-year-old stage 65-year-old stage 68-year-old stage 82-year-old stage 43-year-old stage 41-year-old stage 63-year-old stage 37-year-old stage 58-year-old stage 73-year-old stage 75-year-old stage 84-year-old stage 34-year-old stage 31-year-old stage 59-year-old stage 54-year-old stage 66-year-old stage 61-year-old stage 74-year-old stage 83-year-old stage 89-year-old stage 53-year-old stage 46-year-old stage 56-year-old stage 55-year-old stage 77-year-old stage 94-year-old stage 78-year-old stage 47-year-old stage 32-year-old stage 45-year-old stage 70-year-old stage 81-year-old stage 76-year-old stage 69-year-old stage 67-year-old stage 40-year-old stage 88-year-old stage 90-year-old stage 60-year-old stage 62-year-old stage 39-year-old stage 79-year-old stage 72-year-old stage 27-year-old stage 48-year-old stage 51-year-old stage 71-year-old stage

Normal Myelodysplastic syndrome Hematologic disorder Myelodysplastic/myeloproliferative disease

Female Male

10x 3' v3

Cell

Homo sapiens

Oligodendrocyte

Brodmann (1909) area 13 Brodmann (1909) area 14 Brodmann (1909) area 19 Brodmann (1909) area 23 Brodmann (1909) area 24 Brodmann (1909) area 25 Brodmann (1909) area 32 Brodmann (1909) area 35 Brodmann (1909) area 38 Brodmann (1909) area 40 Brodmann (1909) area 43 Brodmann (1909) area 46 Accessory basal amygdaloid nucleus Agranular insular cortex Anterior cortical amygdaloid nucleus Anterior nuclear group Anterior olfactory nucleus Basolateral amygdaloid nuclear complex Bed nucleus of stria terminalis Body of caudate nucleus Central amygdaloid nucleus Centromedian nucleus of thalamus Cerebellar nuclear complex Cerebellar vermis Cerebrocerebellum Claustrum of brain Corticomedial nuclear complex Cuneus cortex Dentate gyrus of hippocampal formation Dorsal tegmental nucleus Epithalamus Extrastriate cortex Globus pallidus Granular insular cortex Gray matter of midbrain Hippocampal field Hypothalamus Inferior frontal gyrus Inferior olivary complex Inferior temporal gyrus Intralaminar nuclear group Lateral amygdaloid nucleus Lateral entorhinal cortex Lateral geniculate body Lateral nuclear group of thalamus Lateral posterior nucleus of thalamus Mammillary body Medial dorsal nucleus of thalamus Medial entorhinal cortex Medial geniculate body Medial zone of hypothalamus Median tuberal portion Midbrain nucleus Midbrain tectum Middle temporal gyrus Nucleus accumbens Nucleus of dorsal thalamus Nucleus of medulla oblongata Parabrachial nucleus Perirhinal cortex Piriform cortex Pontine nuclear group Pontine reticular formation Pontine tegmentum Posterior parahippocampal gyrus Preoptic area Pretectal region Primary auditory cortex Primary motor cortex Primary somatosensory cortex Primary visual cortex Pulvinar nucleus Putamen Red nucleus Retrosplenial region Rostral ca3 Septal nuclear complex Spinal cord Subicular complex Substantia innominata Substantia nigra Subthalamic nucleus Superior parietal cortex Superior temporal gyrus Supraoptic nucleus Temporal fusiform gyrus Uncal ca1 Ventral anterior nucleus of thalamus

50-year-old stage 29-year-old stage 42-year-old stage 60-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Mature nk t cell Natural killer cell Activated cd8-positive, alpha-beta t cell Endothelial cell of lymphatic vessel Capillary endothelial cell Mast cell Fibroblast Cd14-positive monocyte Monocyte Pericyte Activated cd4-positive, alpha-beta t cell Macrophage Dendritic cell Mesothelial cell Neural cell Vein endothelial cell Cd8-positive, alpha-beta cytotoxic t cell Regular ventricular cardiac myocyte Regular atrial cardiac myocyte Cd4-positive, alpha-beta cytotoxic t cell Cd14-positive, cd16-positive monocyte Endothelial cell Smooth muscle cell Endothelial cell of artery B cell Epicardial adipocyte

Apex of heart Heart left ventricle Heart right ventricle Interventricular septum Left cardiac atrium Right cardiac atrium

Fifth decade stage Eighth decade stage Seventh decade stage Sixth decade stage

Normal

Female Male

10x 3' v2 10x 3' v3

Nucleus Cell