Homo sapiens

Neuron

Choroid plexus epithelium Striatum Medulla oblongata Hindbrain Telencephalon Cortex Midbrain Dorsal plus ventral thalamus Lower layers of the cortex Frontal cortex Entorhinal cortex Midbrain tectum Metencephalon Basal forebrain Cerebellum Brain Hypothalamus Cerebellar peduncular complex Upper layers of the cortex Caudate-putamen Hippocampal formation Diencephalon Pons Cerebral subcortex Occipital cortex Forebrain Head

13th week post-fertilization stage 14th week post-fertilization stage 15th week post-fertilization stage 12th week post-fertilization stage Carnegie stage 20 9th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 22 Carnegie stage 16 Carnegie stage 15 Carnegie stage 18 11th week post-fertilization stage

Normal

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Natural killer cell Cd16-positive, cd56-dim natural killer cell, human Innate lymphoid cell Cd16-negative, cd56-bright natural killer cell, human

Blood

66-year-old stage 69-year-old stage 75-year-old stage 65-year-old stage 42-year-old stage 89-year-old stage 43-year-old stage 87-year-old stage 71-year-old stage 85-year-old stage 53-year-old stage 61-year-old stage 88-year-old stage 52-year-old stage 54-year-old stage 68-year-old stage 48-year-old stage 44-year-old stage 56-year-old stage 55-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 80-year-old stage 51-year-old stage 57-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 80 year-old and over stage 49-year-old stage 58-year-old stage 59-year-old stage 67-year-old stage 77-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 86-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 78-year-old stage 63-year-old stage 47-year-old stage 76-year-old stage 79-year-old stage 73-year-old stage 83-year-old stage 40-year-old stage

Cytomegalovirus infection Normal

Female Male

10x gene expression flex

Cell

Mus musculus

Cardiac valve cell Spinal cord interneuron Premigratory neural crest cell Paraxial cell Notochordal cell Neuroplacodal cell Neurectodermal cell Hemangioblast Mesodermal cell Gut endothelial cell Primitive red blood cell Splanchnic mesodermal cell Surface ectodermal cell Midbrain dopaminergic neuron Endodermal cell Anterior visceral endoderm cell

Embryo

Theiler stage 14

Normal

Slide-seqv2

Mus musculus

Bladder urothelial cell Podocyte Conjunctiva goblet cell Epithelial cell of parathyroid gland Gingival epithelial cell Olfactory epithelial cell Endo-epithelial cell Intermediate mesodermal cell Corneal epithelial cell Pancreatic acinar cell Ectodermal cell Auditory epithelial cell Kidney collecting duct intercalated cell Metanephric mesenchyme stem cell Epithelial cell of amnion Ureteric bud cell Notochordal cell Epithelial cell of proximal tubule Epithelial cell Epithelial cell of thyroid gland Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney connecting tubule epithelial cell Keratinocyte Pituitary gland cell Kidney epithelial cell Lens epithelial cell Kidney loop of henle ascending limb epithelial cell Epithelial cell of thymus Primordial germ cell Intrahepatic cholangiocyte

Embryo

Theiler stage 22 Theiler stage 27 Theiler stage 24 Theiler stage 18 Theiler stage 25 Theiler stage 14 Theiler stage 16 Theiler stage 17 Theiler stage 15 Theiler stage 19 Theiler stage 26 Theiler stage 13 Theiler stage 20 Theiler stage 12 Theiler stage 21 Theiler stage 23

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Periportal region hepatocyte Blood vessel endothelial cell Migratory dendritic cell Hepatocyte Endothelial cell of periportal hepatic sinusoid Natural killer cell Endothelial cell of lymphatic vessel Centrilobular region hepatocyte Kupffer cell Endothelial cell of sinusoid Igg memory b cell Cd8-positive, alpha-beta t cell Exhausted t cell Endothelial cell of hepatic portal vein Endothelial cell of artery Classical monocyte Plasmacytoid dendritic cell Hepatic stellate cell Mature neutrophil Lymphocyte Large mucus secreting cholangiocyte Endothelial cell of pericentral hepatic sinusoid Regulatory t cell Hepatic portal fibroblast Helper t cell Cholangiocyte Trem2-positive macrophage Plasma cell Iga memory b cell Mucosal invariant t cell B cell, cd19-positive Monocyte Vascular associated smooth muscle cell Conventional dendritic cell Dendritic cell Intrahepatic cholangiocyte Bag3-positive monocyte Non-classical monocyte

Left lobe of liver Right lobe of liver Caudate lobe of liver Liver

66-year-old stage 37-year-old stage 75-year-old stage 21-year-old stage 65-year-old stage 26-year-old stage Sixth decade stage 71-year-old stage 53-year-old stage 61-year-old stage 52-year-old stage 54-year-old stage 68-year-old stage 48-year-old stage 39-year-old stage 44-year-old stage 56-year-old stage Fifth decade stage 55-year-old stage 50-year-old stage 80-year-old stage 28-year-old stage 51-year-old stage 57-year-old stage 72-year-old stage Third decade stage 49-year-old stage Seventh decade stage 58-year-old stage 59-year-old stage Adult stage 67-year-old stage Late adult stage 32-year-old stage 77-year-old stage 33-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage Fourth decade stage Eighth decade stage 73-year-old stage Prime adult stage Ninth decade stage Young adult stage

Normal

Female Male

10x 5' v1 10x 3' v3 10x 3' v2

Nucleus Cell

Homo sapiens

Radial glial cell

Choroid plexus epithelium Striatum Medulla oblongata Hindbrain Telencephalon Cortex Midbrain Dorsal plus ventral thalamus Lower layers of the cortex Frontal cortex Entorhinal cortex Midbrain tectum Metencephalon Basal forebrain Cerebellum Brain Hypothalamus Cerebellar peduncular complex Upper layers of the cortex Caudate-putamen Hippocampal formation Diencephalon Pons Cerebral subcortex Occipital cortex Forebrain Head

13th week post-fertilization stage 14th week post-fertilization stage 15th week post-fertilization stage 12th week post-fertilization stage Carnegie stage 20 9th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 22 Carnegie stage 16 Carnegie stage 15 Carnegie stage 18 11th week post-fertilization stage

Normal

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Endothelial cell Hematopoietic precursor cell Lymphocyte Hematopoietic multipotent progenitor cell B cell Monocyte Antibody secreting cell Dendritic cell

Blood

66-year-old stage 69-year-old stage 37-year-old stage 75-year-old stage 34-year-old stage 65-year-old stage 26-year-old stage 89-year-old stage 43-year-old stage 93-year-old stage 71-year-old stage 85-year-old stage 61-year-old stage 54-year-old stage 68-year-old stage 48-year-old stage 30-year-old stage 39-year-old stage 44-year-old stage 38-year-old stage 55-year-old stage 84-year-old stage 92-year-old stage 82-year-old stage 50-year-old stage 90-year-old stage 80-year-old stage 28-year-old stage 51-year-old stage 57-year-old stage 72-year-old stage 81-year-old stage 49-year-old stage 23-year-old stage 58-year-old stage 59-year-old stage 25-year-old stage 67-year-old stage 32-year-old stage 77-year-old stage 33-year-old stage 62-year-old stage 86-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 78-year-old stage 31-year-old stage 36-year-old stage 63-year-old stage 47-year-old stage 76-year-old stage 73-year-old stage 83-year-old stage 40-year-old stage

Hematologic disorder Myelodysplastic/myeloproliferative disease Normal Myelodysplastic syndrome

Female Male

10x 3' v3

Cell

Homo sapiens

Central memory cd4-positive, alpha-beta t cell T cell Cd1c-positive myeloid dendritic cell Mature gamma-delta t cell Natural killer cell Erythrocyte Platelet Cd141-positive myeloid dendritic cell Effector memory cd4-positive, alpha-beta t cell Intermediate monocyte Cd8aa(i) thymocyte Plasmacytoid dendritic cell Basophil mast progenitor cell Cd8-positive, alpha-beta memory t cell Be cell Memory regulatory t cell Naive thymus-derived cd8-positive, alpha-beta t cell Memory b cell Axl+ dendritic cell, human Cd16-positive, cd56-dim natural killer cell, human Innate lymphoid cell Common myeloid progenitor Plasma cell Naive regulatory t cell Memory ccr4-positive regulatory t cell Cd14-low, cd16-positive monocyte Cd4-positive, alpha-beta memory t cell Naive thymus-derived cd4-positive, alpha-beta t cell Common lymphoid progenitor Mucosal invariant t cell Cd8-positive, alpha-beta regulatory t cell Transitional stage b cell Cd14-positive, cd16-negative classical monocyte Central memory cd8-positive, alpha-beta t cell Naive b cell Double negative thymocyte Cd16-negative, cd56-bright natural killer cell, human Effector memory cd8-positive, alpha-beta t cell

Blood

66-year-old stage 43-year-old stage 71-year-old stage 61-year-old stage 52-year-old stage 68-year-old stage 39-year-old stage 55-year-old stage 50-year-old stage 28-year-old stage 51-year-old stage 72-year-old stage 74-year-old stage 58-year-old stage 32-year-old stage 33-year-old stage 64-year-old stage 78-year-old stage 63-year-old stage 76-year-old stage 73-year-old stage 40-year-old stage

Cytomegalovirus infection Normal

Female Male

10x 3' v3

Cell

Drosophila melanogaster

Neuron Prefollicle cell Adult somatic muscle cell of head Outer photoreceptor cell Optic-lobe-associated cortex glial cell Mechanosensory neuron of leg chordotonal organ Transmedullary y neuron tmy8 T2 neuron Adult abdominal pericardial cell T5c neuron Adult lamina marginal glial cell T4c neuron Transmedullary y neuron tmy4 Nociceptive neuron Gamma kenyon cell Indirect flight muscle cell Transmedullary neuron tm3 Subperineurial glial cell Alpha/beta kenyon cell Transmedullary neuron tm1 Fat cell Proximal medullary amacrine neuron pm2 Adult olfactory receptor neuron or88a Cardial cell Adult malpighian tubule principal cell of initial segment Alpha'/beta' kenyon cell Photoreceptor cell r7 Adult malpighian tubule principal cell Tracheolar cell Adult olfactory receptor neuron or67d Lamina wide-field 2 neuron Adult olfactory receptor neuron or47b Lobula columnar neuron lc10 Distal medullary amacrine neuron dm10 T3 neuron Glial cell Somatic precursor cell Adult optic chiasma glial cell Visceral muscle cell Eo sensory organ accessory cell Polar follicle cell Male germline cell Midgut large flat cell Adult renal stem cell Centrifugal neuron c2 Lamina monopolar neuron l4 Leg taste bristle chemosensory neuron Photoreceptor cell r8 Gustatory receptor neuron Hemocyte Dorsal appendage forming follicle cell Ocellar retinula cell Enteroendocrine cell Maxillary palp olfactory receptor neuron Proximal medullary amacrine neuron pm4 Follicle cell Posterior terminal follicle cell Spermatid Adult enterocyte T4a neuron Antennal lobe projection neuron Eye photoreceptor cell Spermatocyte cyst cell Stretch follicle cell Adult midgut enterocyte Copper cell Chordotonal neuron Johnston organ neuron Tyraminergic neuron Lobula columnar neuron lc12 Spermatogonium Epithelial cell Ensheathing glial cell Germline cell Adult alary muscle cell Muscle cell Enterocyte of anterior adult midgut epithelium Transmedullary neuron tm2 Adult malpighian tubule principal cell of lower segment Transmedullary neuron tm9 Leg muscle motor neuron T4d neuron Transmedullary neuron tm20 Transmedullary neuron tm5c Lamina monopolar neuron l2 Distal medullary amacrine neuron dm8a Multidendritic neuron Escort cell Adult tracheocyte Intestinal stem cell Distal medullary amacrine neuron dm9 Lamina wide-field 1 neuron Adult malpighian tubule principal cell of lower ureter Adult lamina epithelial glial cell Distal medullary amacrine neuron dm12 Adult glial cell Medulla intrinsic neuron mi4 Adult neuron Early elongation stage spermatid Cns surface glial cell Ovarian sheath muscle cell Lamina monopolar neuron l3 T5d neuron Embryonic/larval crystal cell Mechanosensory neuron Auditory sensory neuron Distal medullary amacrine neuron dm11 Tormogen cell Head cyst cell Columnar neuron t1 Adult olfactory receptor neuron gr21a/63a Transmedullary y neuron tmy14 Kenyon cell Sensory neuron T4b neuron Oenocyte Mid-late elongation-stage spermatid Main body follicle cell T4 neuron Adult brain perineurial glial cell Enterocyte of posterior adult midgut epithelium Centrifugal neuron c3 Olfactory receptor neuron Adult oenocyte Adult astrocyte-like glial cell T2a neuron Male accessory gland secondary cell Distal medullary amacrine neuron dm3 T5 neuron Adult olfactory receptor neuron ir75d Medulla intrinsic neuron mi15 Labral sense organ mechanosensory neuron Transmedullary y neuron tmy5a Adult malpighian tubule bar-shaped cell of initial segment Dopaminergic neuron Tendon cell Transmedullary neuron tm29 Spermatocyte Gustatory receptor neuron of the labellum T5a neuron Enteroblast Adult dopaminergic pam neuron Medulla intrinsic neuron mi1 Medulla intrinsic neuron mi9 Pigment cell Male accessory gland main cell Octopaminergic neuron Early-mid elongation-stage spermatid Stalk follicle cell T5b neuron Transmedullary neuron tm4 Adult brain cell body glial cell Adult olfactory receptor neuron or92a Lamina monopolar neuron l5 Cell body glial cell Cone cell Adult differentiating enterocyte Lamina monopolar neuron l1 Adult heart ventral longitudinal muscle cell Adult olfactory receptor neuron or65 Adult malpighian tubule stellate cell of main segment Lobula columnar neuron lc17 Adult olfactory receptor neuron or13a Female germline cell

Malpighian tubule Testis Antenna Wing Body proper Insect proboscis Haltere Trachea Leg Ovary Heart Body wall Male reproductive gland Gut Fat body Adult oenocyte Head

Day 3 of adulthood Day 5 of adulthood Day 2 of adulthood Day 1 of adulthood

Normal

Female Male Mixed sex

10x 3' v3

Rattus norvegicus

Cardiac pacemaker cell of sinoatrial node Myocyte of atrioventricular node T cell Brown adipocyte Club cell Cycling t cell Cardiac atrium fibroblast Cardiac endothelial cell Natural killer cell Endothelial cell of lymphatic vessel Non-myelinating schwann cell Eukaryotic cell Endothelial cell Mast cell Cd8-positive, alpha-beta t cell Peripheral nervous system neuron Schwann cell Cardiac neuron Macrophage Myocyte of sinoatrial node Cardiac ventricle fibroblast Mesothelial cell of epicardium Cd14-low, cd16-positive monocyte B cell Pericyte Regular atrial cardiac myocyte Monocyte Vascular associated smooth muscle cell Regular ventricular cardiac myocyte Fibroblast Dendritic cell Fibroblast of cardiac tissue White adipocyte

Normal

10x 3' v2

Homo sapiens

Endothelial cell Hematopoietic precursor cell Lymphocyte Hematopoietic multipotent progenitor cell B cell Monocyte Antibody secreting cell Dendritic cell

Blood

66-year-old stage 69-year-old stage 37-year-old stage 75-year-old stage 91-year-old stage 34-year-old stage 65-year-old stage 26-year-old stage 89-year-old stage 43-year-old stage 71-year-old stage 85-year-old stage 53-year-old stage 61-year-old stage 88-year-old stage 52-year-old stage 54-year-old stage 68-year-old stage 48-year-old stage 39-year-old stage 56-year-old stage 55-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 90-year-old stage 80-year-old stage 51-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 58-year-old stage 59-year-old stage 67-year-old stage 32-year-old stage 77-year-old stage 64-year-old stage 94-year-old stage 45-year-old stage 62-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 78-year-old stage 31-year-old stage 63-year-old stage 47-year-old stage 76-year-old stage 79-year-old stage 73-year-old stage 83-year-old stage 27-year-old stage 40-year-old stage

Hematologic disorder Myelodysplastic/myeloproliferative disease Normal Myelodysplastic syndrome

Female Male

10x 3' v3

Cell

Homo sapiens

Oligodendrocyte

Subthalamic nucleus Medial geniculate body Brodmann (1909) area 25 Lateral amygdaloid nucleus Anterior nuclear group Corticomedial nuclear complex Pontine reticular formation Temporal fusiform gyrus Brodmann (1909) area 19 Centromedian nucleus of thalamus Intralaminar nuclear group Medial dorsal nucleus of thalamus Parabrachial nucleus Primary visual cortex Claustrum of brain Inferior temporal gyrus Rostral ca3 Nucleus of medulla oblongata Red nucleus Subicular complex Pontine tegmentum Pontine nuclear group Globus pallidus Midbrain nucleus Piriform cortex Hippocampal field Lateral nuclear group of thalamus Brodmann (1909) area 13 Middle temporal gyrus Central amygdaloid nucleus Ventral anterior nucleus of thalamus Septal nuclear complex Brodmann (1909) area 35 Cerebellar nuclear complex Median tuberal portion Extrastriate cortex Epithalamus Dorsal tegmental nucleus Brodmann (1909) area 38 Lateral entorhinal cortex Supraoptic nucleus Brodmann (1909) area 32 Medial entorhinal cortex Midbrain tectum Inferior olivary complex Brodmann (1909) area 40 Inferior frontal gyrus Substantia nigra Agranular insular cortex Brodmann (1909) area 43 Nucleus accumbens Cerebrocerebellum Gray matter of midbrain Substantia innominata Anterior cortical amygdaloid nucleus Primary somatosensory cortex Brodmann (1909) area 14 Anterior olfactory nucleus Accessory basal amygdaloid nucleus Pulvinar nucleus Primary auditory cortex Hypothalamus Brodmann (1909) area 23 Primary motor cortex Brodmann (1909) area 24 Brodmann (1909) area 46 Medial zone of hypothalamus Posterior parahippocampal gyrus Basolateral amygdaloid nuclear complex Perirhinal cortex Granular insular cortex Cerebellar vermis Spinal cord Retrosplenial region Cuneus cortex Dentate gyrus of hippocampal formation Lateral posterior nucleus of thalamus Mammillary body Superior temporal gyrus Body of caudate nucleus Uncal ca1 Bed nucleus of stria terminalis Superior parietal cortex Lateral geniculate body Putamen Pretectal region Nucleus of dorsal thalamus Preoptic area

42-year-old stage 29-year-old stage 50-year-old stage 60-year-old stage

Normal

Female Male

10x 3' v3

Nucleus