Homo sapiens

Fibroblast Epithelial cell of lower respiratory tract Alveolar type 1 fibroblast cell Lung pericyte Pulmonary alveolar epithelial cell Multiciliated columnar cell of tracheobronchial tree Multiciliated epithelial cell Elicited macrophage Ionocyte Mast cell Acinar cell Bronchus fibroblast of lung Vein endothelial cell Alveolar macrophage Smooth muscle cell Stromal cell Plasma cell Dendritic cell B cell Tracheobronchial smooth muscle cell Cd1c-positive myeloid dendritic cell Capillary endothelial cell Mucus secreting cell Respiratory tract hillock cell Myofibroblast cell Club cell Hematopoietic stem cell Non-classical monocyte Pulmonary alveolar type 2 cell Plasmacytoid dendritic cell Respiratory basal cell Tracheobronchial serous cell Alveolar adventitial fibroblast Cd8-positive, alpha-beta t cell Pulmonary alveolar type 1 cell Endothelial cell of lymphatic vessel Lung macrophage Natural killer cell Bronchial goblet cell Tracheobronchial goblet cell Conventional dendritic cell Nasal mucosa goblet cell Classical monocyte Mesothelial cell Cd4-positive, alpha-beta t cell T cell Pulmonary artery endothelial cell Brush cell of tracheobronchial tree Serous secreting cell Pulmonary neuroendocrine cell

Nose Lung parenchyma Respiratory airway

54-year-old stage 35-year-old stage 62-year-old stage 27-year-old stage 26-year-old stage 53-year-old stage 21-year-old stage 61-year-old stage 57-year-old stage Sixth decade stage 49-year-old stage 42-year-old stage 36-year-old stage Fourth decade stage 52-year-old stage 40-year-old stage 38-year-old stage 41-year-old stage 59-year-old stage 30-year-old stage 10-year-old stage 55-year-old stage 23-year-old stage 58-year-old stage 75-year-old stage 22-year-old stage 15-year-old stage 18-year-old stage 51-year-old stage 64-year-old stage 68-year-old stage 29-year-old stage 66-year-old stage 33-year-old stage 69-year-old stage 76-year-old stage Fifth decade stage 31-year-old stage 63-year-old stage 25-year-old stage 56-year-old stage 37-year-old stage 46-year-old stage 24-year-old stage 71-year-old stage Seventh decade stage 44-year-old stage 34-year-old stage 47-year-old stage

Normal

Female Male

10x 3' v3 10x 3' v1 10x 5' v2 10x 5' v1 10x 3' v2

Cell

Homo sapiens

Secretory cell Fibroblast Multiciliated epithelial cell Mast cell Vein endothelial cell Lung secretory cell Dendritic cell B cell Circulating fibrocyte Tracheobronchial smooth muscle cell Capillary endothelial cell Myofibroblast cell Pulmonary alveolar type 2 cell Pulmonary alveolar type 1 cell Endothelial cell of lymphatic vessel Macrophage Monocyte Basal cell T cell Nkp46-positive innate lymphoid cell, human Endothelial cell of artery

Nose Lung parenchyma Respiratory airway

54-year-old stage 35-year-old stage 62-year-old stage 27-year-old stage 26-year-old stage 53-year-old stage 21-year-old stage 61-year-old stage 57-year-old stage 49-year-old stage 42-year-old stage 36-year-old stage 52-year-old stage 40-year-old stage 38-year-old stage 41-year-old stage 59-year-old stage 30-year-old stage 10-year-old stage 55-year-old stage 23-year-old stage 58-year-old stage 75-year-old stage 22-year-old stage 15-year-old stage 18-year-old stage 50-year-old stage 64-year-old stage 68-year-old stage 29-year-old stage 66-year-old stage 33-year-old stage 69-year-old stage 32-year-old stage 76-year-old stage 31-year-old stage 63-year-old stage 25-year-old stage 56-year-old stage 37-year-old stage 46-year-old stage 24-year-old stage 67-year-old stage 71-year-old stage 44-year-old stage 34-year-old stage 47-year-old stage

Normal

Female Male

10x 3' v3 10x 3' v1 10x 5' v2 10x 5' v1 10x 3' v2

Cell

Homo sapiens

Fibroblast Schwann cell Mast cell Keratocyte Myelinating schwann cell Smooth muscle cell Melanocyte Epithelial cell Conjunctival epithelial cell B cell Capillary endothelial cell Pericyte Lymphocyte Endothelial cell of venule Endothelial cell of lymphatic vessel Macrophage Monocyte Corneal endothelial cell Endothelial cell of artery

Tunica fibrosa of eyeball Corneal endothelium Cornea Sclera Ocular surface region Corneal epithelium Corneo-scleral junction Substantia propria of cornea

39-year-old stage 62-year-old stage 27-year-old stage 74-year-old stage 72-year-old stage 53-year-old stage 83-year-old stage Postnatal stage 61-year-old stage 13-year-old stage Newborn stage (0-28 days) 1-year-old stage 86-year-old stage 48-year-old stage 70-year-old stage 45-year-old stage 41-year-old stage Eighth decade stage 10-year-old stage 55-year-old stage 2-year-old stage 75-year-old stage 90 year-old and over stage 22-year-old stage 51-year-old stage 64-year-old stage 77-year-old stage 11-year-old stage 33-year-old stage 69-year-old stage 32-year-old stage 87-year-old stage 84-year-old stage 25-year-old stage 67-year-old stage 43-year-old stage 65-year-old stage 34-year-old stage 16-year-old stage

Normal

Female Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Gabaergic amacrine cell Amacrine cell Glycinergic amacrine cell Starburst amacrine cell

Peripheral region of retina Fovea centralis Macula lutea proper Macula lutea

62-year-old stage 27-year-old stage 78-year-old stage 80 year-old and over stage 73-year-old stage 74-year-old stage 72-year-old stage 53-year-old stage 21-year-old stage 83-year-old stage 85-year-old stage 81-year-old stage 82-year-old stage 17-year-old stage 52-year-old stage 86-year-old stage 70-year-old stage 41-year-old stage Eighth decade stage 3-year-old stage 30-year-old stage 10-year-old stage 58-year-old stage 75-year-old stage 90 year-old and over stage 15-year-old stage 18-year-old stage 50-year-old stage 64-year-old stage 68-year-old stage 66-year-old stage 77-year-old stage 11-year-old stage Late adult stage 69-year-old stage 32-year-old stage 76-year-old stage 88-year-old stage 63-year-old stage 60-year-old stage 80-year-old stage Prime adult stage 84-year-old stage 25-year-old stage 46-year-old stage 24-year-old stage 67-year-old stage 43-year-old stage 60-79 year-old stage 65-year-old stage 71-year-old stage 34-year-old stage 16-year-old stage 47-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Drosophila melanogaster

Enterocyte of anterior adult midgut epithelium Maxillary palp olfactory receptor neuron Eo sensory organ accessory cell Mechanosensory neuron of leg chordotonal organ Adult brain cell body glial cell Ovarian sheath muscle cell Transmedullary neuron tm20 Sensory neuron Adult olfactory receptor neuron gr21a/63a Cardial cell Adult abdominal pericardial cell T2a neuron Lamina monopolar neuron l5 Adult midgut enterocyte Medulla intrinsic neuron mi1 Adult malpighian tubule principal cell of initial segment Proximal medullary amacrine neuron pm2 Tormogen cell Transmedullary neuron tm4 Dopaminergic neuron Transmedullary y neuron tmy8 Kenyon cell Transmedullary neuron tm1 Lamina monopolar neuron l2 Spermatogonium Early-mid elongation-stage spermatid Medulla intrinsic neuron mi15 Follicle cell Photoreceptor cell r8 Gustatory receptor neuron Transmedullary neuron tm5c Distal medullary amacrine neuron dm3 Outer photoreceptor cell Adult malpighian tubule principal cell of lower ureter Male accessory gland secondary cell Ocellar retinula cell Adult differentiating enterocyte Medulla intrinsic neuron mi4 Cns surface glial cell Transmedullary neuron tm2 Escort cell Copper cell Auditory sensory neuron Spermatocyte T2 neuron Alpha'/beta' kenyon cell Multidendritic neuron Stretch follicle cell Adult olfactory receptor neuron or67d Johnston organ neuron Adult enterocyte Hemocyte Adult astrocyte-like glial cell Transmedullary y neuron tmy14 Adult olfactory receptor neuron or13a Ensheathing glial cell Alpha/beta kenyon cell Proximal medullary amacrine neuron pm4 Adult malpighian tubule principal cell Antennal lobe projection neuron Subperineurial glial cell Cone cell Adult olfactory receptor neuron or92a Mid-late elongation-stage spermatid Leg taste bristle chemosensory neuron Germline cell Lamina wide-field 1 neuron Visceral muscle cell Adult malpighian tubule stellate cell of main segment Dorsal appendage forming follicle cell Adult dopaminergic pam neuron Intestinal stem cell Centrifugal neuron c3 Adult olfactory receptor neuron or88a Spermatid Polar follicle cell Nociceptive neuron Medulla intrinsic neuron mi9 Pigment cell Lobula columnar neuron lc12 Transmedullary neuron tm9 Lamina wide-field 2 neuron Lobula columnar neuron lc17 Stalk follicle cell Cell body glial cell Transmedullary y neuron tmy4 Adult brain perineurial glial cell Chordotonal neuron Indirect flight muscle cell Adult glial cell Adult neuron Epithelial cell Adult oenocyte Adult olfactory receptor neuron or65 Adult heart ventral longitudinal muscle cell Enterocyte of posterior adult midgut epithelium Tendon cell Labral sense organ mechanosensory neuron Male accessory gland main cell Enteroblast Distal medullary amacrine neuron dm10 Lobula columnar neuron lc10 Distal medullary amacrine neuron dm8a Adult alary muscle cell Transmedullary neuron tm3 Distal medullary amacrine neuron dm12 Adult optic chiasma glial cell Embryonic/larval crystal cell Optic-lobe-associated cortex glial cell Transmedullary y neuron tmy5a T3 neuron Lamina monopolar neuron l1 Early elongation stage spermatid Eye photoreceptor cell Muscle cell Leg muscle motor neuron Adult lobula astrocyte-like glial cell Olfactory receptor neuron Lamina monopolar neuron l4 Distal medullary amacrine neuron dm9 Midgut large flat cell Adult olfactory receptor neuron ir75d Enteroendocrine cell Adult malpighian tubule bar-shaped cell of initial segment Adult olfactory receptor neuron or47b Columnar neuron t1 Adult renal stem cell Adult malpighian tubule principal cell of lower segment Centrifugal neuron c2 Adult somatic muscle cell of head Distal medullary amacrine neuron dm11 Gustatory receptor neuron of the labellum Gamma kenyon cell Photoreceptor cell r7 Lamina monopolar neuron l3 Mechanosensory neuron

Leg Trachea Gut Malpighian tubule Wing Body wall Male reproductive gland Antenna Proboscis Oenocyte Fat body Heart Haltere Ovary Testis Maxillary palp Head

Day 2 of adulthood Day 1 of adulthood Day 5 of adulthood Day 3 of adulthood

Normal

Mixed sex Female Male

10x 3' v3

Homo sapiens

Fibroblast Cytotoxic t cell Innate lymphoid cell Helper t cell Schwann cell Mast cell Endothelial cell of arteriole Vein endothelial cell Myelinating schwann cell Smooth muscle cell Melanocyte Plasma cell Dendritic cell B cell Non-myelinating schwann cell Megakaryocyte Vascular associated smooth muscle cell Retinal pigment epithelial cell Pericyte Non-classical monocyte Plasmacytoid dendritic cell Endothelial cell of venule Natural killer cell Macrophage Fenestrated endothelial cell Classical monocyte T cell Endothelial cell of artery

Chorioretinal region

62-year-old stage 27-year-old stage 72-year-old stage 53-year-old stage 21-year-old stage 81-year-old stage Newborn stage (0-28 days) 82-year-old stage 17-year-old stage 86-year-old stage 30-year-old stage 10-year-old stage 58-year-old stage 90 year-old and over stage 50-year-old stage 64-year-old stage 68-year-old stage 77-year-old stage 11-year-old stage 69-year-old stage 32-year-old stage 63-year-old stage 60-year-old stage 25-year-old stage 46-year-old stage 43-year-old stage 65-year-old stage 71-year-old stage 34-year-old stage 16-year-old stage 47-year-old stage

Normal

Female Male

10x 3' v3 10x 5' v2

Nucleus

Homo sapiens

Neuron

Midbrain Midbrain tectum Striatum Cortex Hypothalamus Cerebral subcortex Metencephalon Basal forebrain Brain Forebrain Pons Cerebellum Entorhinal cortex Telencephalon Hippocampal formation Occipital cortex Diencephalon Choroid plexus epithelium Upper layers of the cortex Hindbrain Caudate-putamen Lower layers of the cortex Cerebellar peduncular complex Frontal cortex Medulla oblongata Head Dorsal plus ventral thalamus

Carnegie stage 20 Carnegie stage 18 14th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 22 Carnegie stage 15 15th week post-fertilization stage 11th week post-fertilization stage 13th week post-fertilization stage 12th week post-fertilization stage 9th week post-fertilization stage Carnegie stage 16

Normal

10x 3' v3 10x 3' v2

Cell

Mus musculus

Primitive red blood cell Premigratory neural crest cell Neurectodermal cell Cardiac valve cell Endodermal cell Spinal cord interneuron Gut endothelial cell Splanchnic mesodermal cell Midbrain dopaminergic neuron Hemangioblast Anterior visceral endoderm cell Mesodermal cell Neuroplacodal cell Surface ectodermal cell Notochordal cell Paraxial cell

Embryo

Theiler stage 14

Normal

Slide-seqv2

Mus musculus

Pituitary gland cell Kidney loop of henle ascending limb epithelial cell Keratinocyte Pancreatic acinar cell Epithelial cell of thyroid gland Epithelial cell of proximal tubule Podocyte Kidney connecting tubule epithelial cell Endo-epithelial cell Ureteric bud cell Olfactory epithelial cell Epithelial cell Primordial germ cell Metanephric mesenchyme stem cell Intrahepatic cholangiocyte Intermediate mesodermal cell Kidney epithelial cell Epithelial cell of parathyroid gland Epithelial cell of thymus Kidney distal convoluted tubule epithelial cell Bladder urothelial cell Kidney collecting duct intercalated cell Gingival epithelial cell Conjunctiva goblet cell Corneal epithelial cell Epithelial cell of amnion Kidney collecting duct principal cell Ectodermal cell Notochordal cell Auditory epithelial cell Lens epithelial cell

Embryo

Theiler stage 26 Theiler stage 18 Theiler stage 13 Theiler stage 24 Theiler stage 15 Theiler stage 14 Theiler stage 12 Theiler stage 23 Theiler stage 20 Theiler stage 22 Theiler stage 16 Theiler stage 21 Theiler stage 25 Theiler stage 27 Theiler stage 17 Theiler stage 19

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Radial glial cell

Midbrain Midbrain tectum Striatum Cortex Hypothalamus Cerebral subcortex Metencephalon Basal forebrain Brain Forebrain Pons Cerebellum Entorhinal cortex Telencephalon Hippocampal formation Occipital cortex Diencephalon Choroid plexus epithelium Upper layers of the cortex Hindbrain Caudate-putamen Lower layers of the cortex Cerebellar peduncular complex Frontal cortex Medulla oblongata Head Dorsal plus ventral thalamus

Carnegie stage 20 Carnegie stage 18 14th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 22 Carnegie stage 15 15th week post-fertilization stage 11th week post-fertilization stage 13th week post-fertilization stage 12th week post-fertilization stage 9th week post-fertilization stage Carnegie stage 16

Normal

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Hematopoietic multipotent progenitor cell Antibody secreting cell Hematopoietic precursor cell Dendritic cell B cell Lymphocyte Monocyte Endothelial cell

Blood

54-year-old stage 90-year-old stage 39-year-old stage 62-year-old stage 78-year-old stage 73-year-old stage 26-year-old stage 72-year-old stage 83-year-old stage 85-year-old stage 81-year-old stage 61-year-old stage 57-year-old stage 82-year-old stage 49-year-old stage 36-year-old stage 86-year-old stage 48-year-old stage 70-year-old stage 40-year-old stage 38-year-old stage 41-year-old stage 59-year-old stage 30-year-old stage 55-year-old stage 23-year-old stage 58-year-old stage 75-year-old stage 50-year-old stage 51-year-old stage 89-year-old stage 68-year-old stage 66-year-old stage 77-year-old stage 33-year-old stage 93-year-old stage 69-year-old stage 32-year-old stage 28-year-old stage 76-year-old stage 31-year-old stage 63-year-old stage 60-year-old stage 80-year-old stage 84-year-old stage 25-year-old stage 37-year-old stage 46-year-old stage 92-year-old stage 67-year-old stage 43-year-old stage 65-year-old stage 71-year-old stage 44-year-old stage 34-year-old stage 47-year-old stage

Hematologic disorder Myelodysplastic/myeloproliferative disease Normal Myelodysplastic syndrome

Female Male

10x 3' v3

Cell

Drosophila melanogaster

Eo sensory organ accessory cell Ovarian sheath muscle cell Sensory neuron Follicle cell Escort cell Hemocyte Subperineurial glial cell Germline cell Visceral muscle cell Cell body glial cell Adult brain perineurial glial cell Indirect flight muscle cell Epithelial cell Adult heart ventral longitudinal muscle cell Adult alary muscle cell Embryonic/larval crystal cell Muscle cell Enteroendocrine cell Adult renal stem cell Adult somatic muscle cell of head Mechanosensory neuron

Leg Trachea Adult oenocyte Gut Malpighian tubule Insect proboscis Body proper Wing Body wall Male reproductive gland Antenna Fat body Heart Haltere Ovary Testis Head

Day 2 of adulthood Day 1 of adulthood Day 5 of adulthood Day 3 of adulthood

Normal

Female Male Mixed sex

10x 3' v3