Homo sapiens

Kidney loop of henle thin descending limb epithelial cell Kidney loop of henle thick ascending limb epithelial cell Renal interstitial pericyte Mucosal invariant t cell Parietal epithelial cell Kidney resident macrophage Mast cell Effector memory cd8-positive, alpha-beta t cell Cd8-alpha-positive thymic conventional dendritic cell Epithelial cell of proximal tubule Podocyte Kidney connecting tubule epithelial cell Plasma cell B cell Naive thymus-derived cd4-positive, alpha-beta t cell Non-classical monocyte Plasmacytoid dendritic cell, human Migratory dendritic cell Kidney interstitial fibroblast Kidney loop of henle thin ascending limb epithelial cell Neural cell Kidney distal convoluted tubule epithelial cell Effector memory cd8-positive, alpha-beta t cell, terminally differentiated Natural killer cell Macrophage Kidney collecting duct intercalated cell Cycling macrophage Group 3 innate lymphoid cell Classical monocyte Cd8-alpha-negative thymic conventional dendritic cell Kidney collecting duct principal cell Naive regulatory t cell Endothelial cell

Kidney

35-year-old stage 39-year-old stage Ninth decade stage Third decade stage 57-year-old stage Sixth decade stage 49-year-old stage 42-year-old stage Fourth decade stage 52-year-old stage 48-year-old stage 40-year-old stage 41-year-old stage Eighth decade stage 30-year-old stage 66-year-old stage Fifth decade stage 31-year-old stage 56-year-old stage Seventh decade stage 47-year-old stage

Chronic kidney disease Acute kidney failure Normal

Female Male

10x 3' v3

Cell

Homo sapiens

Secretory cell Alveolar type 1 fibroblast cell Innate lymphoid cell Helper t cell Mucus secreting cell of tracheobronchial tree submucosal gland Mesothelial cell of pleura Airway submucosal gland collecting duct epithelial cell Ionocyte Secondary crest myofibroblast Mast cell Vein endothelial cell Alveolar macrophage Lung secretory cell Neutrophil Plasma cell B cell Regulatory t cell Lung megakaryocyte Chondrocyte Tracheobronchial smooth muscle cell Myoepithelial cell Vascular associated smooth muscle cell Alveolar capillary type 2 endothelial cell Pericyte Mature conventional dendritic cell Alveolar capillary type 1 endothelial cell Non-classical monocyte Pulmonary alveolar type 2 cell Plasmacytoid dendritic cell Goblet cell Alveolar adventitial fibroblast Pulmonary alveolar type 1 cell Natural killer cell Deuterosomal cell Conventional dendritic cell Ciliated cell Basal cell Lung interstitial macrophage Classical monocyte Vein endothelial cell of respiratory system Respiratory tract suprabasal cell Endothelial cell of respiratory system lymphatic vessel Brush cell of tracheobronchial tree Serous secreting cell Cd8-positive, alpha-beta cytotoxic t cell Pulmonary neuroendocrine cell Endothelial cell of artery

Lung

54-year-old stage 35-year-old stage 39-year-old stage 62-year-old stage 27-year-old stage 26-year-old stage 72-year-old stage 21-year-old stage 61-year-old stage 57-year-old stage 13-year-old stage 49-year-old stage 42-year-old stage 36-year-old stage 17-year-old stage 52-year-old stage 48-year-old stage 70-year-old stage 40-year-old stage 12-year-old stage 38-year-old stage 45-year-old stage 41-year-old stage 59-year-old stage 30-year-old stage 10-year-old stage 55-year-old stage 23-year-old stage 75-year-old stage 22-year-old stage 50-year-old stage 51-year-old stage 79-year-old stage 64-year-old stage 68-year-old stage 29-year-old stage 66-year-old stage 33-year-old stage 69-year-old stage 32-year-old stage 76-year-old stage 31-year-old stage 63-year-old stage 80-year-old stage 25-year-old stage 56-year-old stage 37-year-old stage 46-year-old stage 24-year-old stage 67-year-old stage 20-year-old stage 65-year-old stage 44-year-old stage 47-year-old stage

Normal

Female Male

10x 3' v3 10x 5' v1 10x 3' v2

Cell

Mus musculus

Astrocyte Inhibitory interneuron Cell Oligodendrocyte precursor cell Oligodendrocyte Pericyte Macrophage Medium spiny neuron Vascular leptomeningeal cell Microglial cell T cell Neuron Endothelial cell

Cortical layer vi Striatum Cortical layer v Olfactory region Pia mater Cortical layer ii/iii Corpus callosum Brain ventricle

24-week-old stage 4-week-old stage 20-month-old stage and over

Normal

Female

Merfish

Homo sapiens

Retinal ganglion cell Off parasol ganglion cell On midget ganglion cell On parasol ganglion cell Off midget ganglion cell

Peripheral region of retina Fovea centralis Macula lutea proper Macula lutea

62-year-old stage 27-year-old stage 78-year-old stage 80 year-old and over stage 73-year-old stage 74-year-old stage 72-year-old stage 53-year-old stage 21-year-old stage 83-year-old stage 85-year-old stage 81-year-old stage 82-year-old stage 17-year-old stage 52-year-old stage 86-year-old stage 70-year-old stage 41-year-old stage Eighth decade stage 3-year-old stage 30-year-old stage 10-year-old stage 58-year-old stage 75-year-old stage 90 year-old and over stage 15-year-old stage 18-year-old stage 50-year-old stage 64-year-old stage 68-year-old stage 66-year-old stage 77-year-old stage 11-year-old stage Late adult stage 69-year-old stage 32-year-old stage 76-year-old stage 88-year-old stage 63-year-old stage 60-year-old stage 80-year-old stage Prime adult stage 84-year-old stage 25-year-old stage 46-year-old stage 67-year-old stage 43-year-old stage 60-79 year-old stage 65-year-old stage 71-year-old stage 34-year-old stage 16-year-old stage 47-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

L2/3-6 intratelencephalic projecting glutamatergic neuron

Dorsolateral prefrontal cortex

78-year-old stage 80 year-old and over stage 72-year-old stage 83-year-old stage 85-year-old stage 81-year-old stage 82-year-old stage 42-year-old stage 86-year-old stage 70-year-old stage 75-year-old stage 50-year-old stage 89-year-old stage 68-year-old stage 29-year-old stage 77-year-old stage 69-year-old stage 88-year-old stage 87-year-old stage 80-year-old stage 84-year-old stage

Dementia Normal

Female Male

10x multiome 10x 3' v3

Nucleus

Homo sapiens

Astrocyte Mast cell Mural cell Oligodendrocyte precursor cell Malignant cell Plasma cell Oligodendrocyte Dendritic cell B cell Mature t cell Natural killer cell Macrophage Radial glial cell Monocyte Microglial cell Neuron Endothelial cell

Right temporal lobe Left frontal lobe Right parietal lobe Temporal lobe Brain Forebrain Left temporal lobe Left parietal lobe Temporoparietal junction Frontal lobe Right frontal lobe

54-year-old stage Adult stage 62-year-old stage 78-year-old stage 74-year-old stage 72-year-old stage 53-year-old stage 21-year-old stage 83-year-old stage 61-year-old stage 57-year-old stage 42-year-old stage 36-year-old stage 52-year-old stage 48-year-old stage 70-year-old stage 45-year-old stage 59-year-old stage 55-year-old stage 58-year-old stage 50-year-old stage 51-year-old stage 64-year-old stage 68-year-old stage 66-year-old stage 77-year-old stage 33-year-old stage 69-year-old stage 76-year-old stage 63-year-old stage 60-year-old stage 80-year-old stage 56-year-old stage 46-year-old stage 67-year-old stage 65-year-old stage 71-year-old stage 44-year-old stage 34-year-old stage

Glioblastoma

Female Male

Microwell-seq Bd rhapsody whole transcriptome analysis Cel-seq2 10x 3' v3 Strt-seq Smart-seq2 10x 5' v1 10x 3' v2

Cell

Homo sapiens

Plasmablast Effector memory cd4-positive, alpha-beta t cell Class switched memory b cell Mucosal invariant t cell Hematopoietic multipotent progenitor cell Cd16-positive, cd56-dim natural killer cell, human Effector memory cd8-positive, alpha-beta t cell Unswitched memory b cell Central memory cd4-positive, alpha-beta t cell Memory b cell Plasma cell Gamma-delta t cell Regulatory t cell Megakaryocyte Effector memory cd4-positive, alpha-beta t cell, terminally differentiated Non-classical monocyte Plasmacytoid dendritic cell Leukocyte Effector memory cd8-positive, alpha-beta t cell, terminally differentiated Cd16-negative, cd56-bright natural killer cell, human Central memory cd8-positive, alpha-beta t cell Conventional dendritic cell Classical monocyte Naive b cell

Blood

35-year-old stage Adult stage 39-year-old stage 62-year-old stage 27-year-old stage 73-year-old stage 26-year-old stage 53-year-old stage 21-year-old stage 57-year-old stage Sixth decade stage 49-year-old stage 70-year-old stage 40-year-old stage 38-year-old stage 45-year-old stage 59-year-old stage Eighth decade stage 30-year-old stage 55-year-old stage 58-year-old stage 22-year-old stage 51-year-old stage 64-year-old stage 29-year-old stage 33-year-old stage Late adult stage 69-year-old stage 32-year-old stage 28-year-old stage 63-year-old stage 37-year-old stage 46-year-old stage 71-year-old stage Seventh decade stage 44-year-old stage 34-year-old stage

Normal

Female Male

10x 3' v3 10x 5' v2 10x 5' v1 10x 3' v2

Cell

Homo sapiens

Fibroblast Myeloid cell Neutrophil Malignant cell Plasma cell B cell Megakaryocyte Erythroid lineage cell Megakaryocyte-erythroid progenitor cell Natural killer cell Macrophage T cell

Pleural fluid Blood Bone marrow

Nursing stage (0-11 months) Pediatric stage

T-cell childhood acute lymphocytic leukemia

Female Male

10x 5' v2

Cell

Homo sapiens

Fibroblast Pigmented ciliary epithelial cell Schwann cell Mast cell Myelinating schwann cell Melanocyte B cell Blood vessel endothelial cell Trabecular meshwork cell Pericyte Lymphocyte Macrophage Non-pigmented ciliary epithelial cell Ciliary muscle cell Endothelial cell

Eye trabecular meshwork Ciliary body

62-year-old stage 27-year-old stage 78-year-old stage 73-year-old stage 74-year-old stage 72-year-old stage 21-year-old stage 85-year-old stage 61-year-old stage Newborn stage (0-28 days) 1-year-old stage 70-year-old stage 45-year-old stage 55-year-old stage 2-year-old stage 75-year-old stage 51-year-old stage 64-year-old stage 68-year-old stage 11-year-old stage 69-year-old stage 32-year-old stage 60-year-old stage 56-year-old stage 43-year-old stage 65-year-old stage 34-year-old stage 16-year-old stage

Normal

Female Male

10x 3' v3 10x 5' v2 10x 3' v2

Cell

Mus musculus

Type 2 cone bipolar cell (sensu mus) Retinal ganglion cell Type 1 cone bipolar cell (sensu mus) Retinal rod cell Astrocyte Type 5b cone bipolar cell Type 4 cone bipolar cell (sensu mus) Type 9 cone bipolar cell (sensu mus) Gabaergic amacrine cell Amacrine cell Retina horizontal cell Retinal bipolar neuron Type 7 cone bipolar cell (sensu mus) Type 5a cone bipolar cell Type 3b cone bipolar cell Type 6 cone bipolar cell (sensu mus) Retinal pigment epithelial cell Glycinergic amacrine cell Pericyte Type 8 cone bipolar cell (sensu mus) Rod bipolar cell Retinal cone cell Mueller cell Type 5 cone bipolar cell (sensu mus) Microglial cell Type 3a cone bipolar cell Endothelial cell

Normal

10x 3' v3 Drop-seq 10x 3' v2

Mus musculus

Type 2 cone bipolar cell (sensu mus) Retinal ganglion cell Type 1 cone bipolar cell (sensu mus) Retinal rod cell Astrocyte Type 5b cone bipolar cell Type 4 cone bipolar cell (sensu mus) Type 9 cone bipolar cell (sensu mus) Gabaergic amacrine cell Amacrine cell Retina horizontal cell Retinal bipolar neuron Type 7 cone bipolar cell (sensu mus) Type 5a cone bipolar cell Type 3b cone bipolar cell Type 6 cone bipolar cell (sensu mus) Retinal pigment epithelial cell Glycinergic amacrine cell Pericyte Type 8 cone bipolar cell (sensu mus) Rod bipolar cell Retinal cone cell Mueller cell Type 5 cone bipolar cell (sensu mus) Microglial cell Type 3a cone bipolar cell Endothelial cell

Retina Optic cup

12-week-old stage 11-week-old stage 8-week-old stage 5-week-old stage 7-week-old stage 9-week-old stage Postnatal stage 4-month-old stage Prime adult stage 2-week-old stage 25-week-old stage

Normal

Male

10x 3' v3 Drop-seq 10x 3' v2

Cell

Homo sapiens

L2/3-6 intratelencephalic projecting glutamatergic neuron

Middle temporal gyrus

Adult stage 78-year-old stage 80 year-old and over stage 72-year-old stage 83-year-old stage 85-year-old stage 81-year-old stage 82-year-old stage 86-year-old stage 70-year-old stage 75-year-old stage 50-year-old stage 89-year-old stage 68-year-old stage 29-year-old stage 77-year-old stage 69-year-old stage 88-year-old stage 87-year-old stage 60-year-old stage 80-year-old stage 84-year-old stage 43-year-old stage 65-year-old stage

Dementia Normal

Female Male

10x multiome 10x 3' v3

Nucleus