Homo sapiens

Central memory cd4-positive, alpha-beta t cell Neutrophil Dendritic cell, human Erythrocyte Gamma-delta t cell Endothelial cell Mast cell Effector memory cd4-positive, alpha-beta t cell Erythroid lineage cell Leukocyte Classical monocyte Plasmacytoid dendritic cell Macrophage Effector memory cd8-positive, alpha-beta t cell, terminally differentiated Memory b cell Cd16-positive, cd56-dim natural killer cell, human Innate lymphoid cell Regulatory t cell Plasmablast Plasma cell Hematopoietic multipotent progenitor cell Megakaryocyte T follicular helper cell B cell Mucosal invariant t cell Central memory cd8-positive, alpha-beta t cell Naive b cell Conventional dendritic cell Cd16-negative, cd56-bright natural killer cell, human Effector memory cd8-positive, alpha-beta t cell Non-classical monocyte

Spleen

69-year-old stage Sixth decade stage 61-year-old stage Fifth decade stage Third decade stage Seventh decade stage 59-year-old stage 31-year-old stage Eighth decade stage

Normal

Female Male

10x 3' v2 10x 3' v3 10x 5' transcription profiling 10x 5' v1 10x 5' v2 Smart-seq2

Cell

Mus musculus

Neutrophil Cycling t cell Blood vessel endothelial cell Migratory dendritic cell Natural killer cell Fibroblast of mammary gland Perivascular cell Mast cell Mammary gland epithelial cell Cd8-positive, alpha-beta t cell Schwann cell Plasmacytoid dendritic cell Macrophage Cd4-positive, cd25-positive, alpha-beta regulatory t cell Lactocyte Luminal adaptive secretory precursor cell of mammary gland Innate lymphoid cell Basal-myoepithelial cell of mammary gland Plasma cell Cd4-positive, alpha-beta t cell Luminal hormone-sensing cell of mammary gland B cell Myeloid leukocyte Conventional dendritic cell

Mammary gland

11-month-old stage 10-month-old stage 12-month-old stage 3-month-old stage 9-month-old stage 7-month-old stage 18-month-old stage 8-month-old stage 13-month-old stage

Normal Neoplasm

Female

10x 3' v2 10x 3' v3

Cell

Mus musculus

T cell Neutrophil Endothelial cell of lymphatic vessel Endothelial cell Mature nk t cell Mast cell Mammary gland epithelial cell Smooth muscle cell Epithelial cell Preadipocyte Adipocyte Macrophage Mesothelial cell B cell Pericyte Monocyte Dendritic cell

Periovarian fat pad Inguinal fat pad Epididymal fat pad

19-week-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

T cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Mast cell Ependymal cell Macrophage Microglial cell Interneuron Choroid plexus epithelial cell B cell Pericyte Neuron Astrocyte Fibroblast

Normal Alzheimer disease

10x 3' v3

Homo sapiens

Natural killer cell Endothelial cell of lymphatic vessel Cytotoxic t cell Mast cell Inflammatory macrophage Schwann cell Melanocyte Endothelial cell of vascular tree Macrophage Innate lymphoid cell Regulatory t cell Skin fibroblast Monocyte-derived dendritic cell Helper t cell Keratinocyte Plasma cell Langerhans cell Pericyte Conventional dendritic cell Dendritic cell

Dermis Skin epidermis

Adult stage

Normal

Female

10x 3' v2

Cell

Homo sapiens

Endothelial cell of lymphatic vessel Endothelial cell Endothelial cell of artery Capillary endothelial cell Smooth muscle cell Vein endothelial cell Mesothelial cell Pericyte

Heart right ventricle Right cardiac atrium Apex of heart Heart left ventricle Left cardiac atrium Interventricular septum

Sixth decade stage Fifth decade stage Seventh decade stage Eighth decade stage

Normal

Female Male

10x 3' v2 10x 3' v3

Nucleus Cell

Homo sapiens

T cell Podocyte Dendritic cell, human Natural killer cell Endothelial cell of lymphatic vessel Parietal epithelial cell Kidney loop of henle thin descending limb epithelial cell Kidney interstitial fibroblast Peritubular capillary endothelial cell Kidney collecting duct intercalated cell Renal medullary fibroblast Classical monocyte Epithelial cell of proximal tubule Kidney inner medulla collecting duct epithelial cell Lymphocyte Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney connecting tubule epithelial cell Mural cell Kidney loop of henle thick ascending limb epithelial cell Myeloid cell Vasa recta cell Kidney arterial blood vessel cell B cell Vascular associated smooth muscle cell Kidney loop of henle thin ascending limb epithelial cell Glomerular capillary endothelial cell Conventional dendritic cell Non-classical monocyte

Renal medulla Renal pelvis Renal papilla Kidney Cortex of kidney

66-year-old stage 69-year-old stage 42-year-old stage 43-year-old stage 71-year-old stage 53-year-old stage 61-year-old stage 52-year-old stage 54-year-old stage 68-year-old stage 48-year-old stage 30-year-old stage 39-year-old stage 44-year-old stage 29-year-old stage 56-year-old stage 50-year-old stage 57-year-old stage 72-year-old stage 74-year-old stage 49-year-old stage 35-year-old stage Seventh decade stage 58-year-old stage 59-year-old stage 67-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 31-year-old stage 63-year-old stage Eighth decade stage 47-year-old stage 76-year-old stage 40-year-old stage

Normal

Female Male

10x 3' v2 10x 3' v3 10x 5' v1

Nucleus Cell

Mus musculus

T cell Neutrophil Endocardial cell Granulocyte Hepatocyte Exocrine cell Natural killer cell Kidney tubule cell Endothelial cell Kupffer cell Mature nk t cell Pancreatic pp cell Schwann cell Hepatic stellate cell Pancreatic a cell Macrophage Pancreatic d cell Type b pancreatic cell Cardiac muscle cell B cell Pericyte Circulating fibrocyte Monocyte Fibroblast Dendritic cell

Liver Kidney Heart Pancreas Blood

2-month-old stage

Normal Myocardial infarction

Male

10x 3' v3

Cell

Homo sapiens

Club cell Airway submucosal gland duct basal cell Blood vessel endothelial cell Ciliated cell Natural killer cell Endothelial cell of lymphatic vessel Lung secretory cell Erythrocyte Non-myelinating schwann cell Gamma-delta t cell Neuroendocrine cell Mast cell Lung interstitial macrophage Iga plasma cell Blood vessel smooth muscle cell Lung resident memory cd8-positive, alpha-beta t cell Basal cell Endothelial cell of artery Smooth muscle cell Epithelial cell Smooth muscle cell of the pulmonary artery Plasmacytoid dendritic cell Igg plasma cell Effector cd4-positive, alpha-beta t cell Bronchus fibroblast of lung Myoepithelial cell Lung macrophage Goblet cell Lung resident memory cd4-positive, alpha-beta t cell Memory b cell Innate lymphoid cell Regulatory t cell Deuterosomal cell Pulmonary alveolar type 1 cell Metallothionein-positive alveolar macrophage Plasmablast Serous secreting cell of bronchus submucosal gland Fibroblast of lung Alveolar macrophage Vein endothelial cell Cd14-positive monocyte Megakaryocyte Respiratory tract suprabasal cell Lung perichondrial fibroblast Mesothelial cell Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Pulmonary artery endothelial cell Adventitial cell Monocyte Naive b cell Myofibroblast cell Conventional dendritic cell Fibroblast Myelinating schwann cell Dendritic cell Pulmonary alveolar type 2 cell Effector memory cd8-positive, alpha-beta t cell Lung pericyte Chondrocyte

Bronchus Lung Trachea Upper lobe of left lung Lower lobe of left lung

66-year-old stage 75-year-old stage Sixth decade stage 29-year-old stage 35-year-old stage Seventh decade stage 58-year-old stage 59-year-old stage 64-year-old stage

Normal

Female Male

10x 3' v2 10x 3' v3 10x 5' transcription profiling

Nucleus Cell

Mus musculus

Neutrophil Endothelial cell Smooth muscle cell Connective tissue cell Macrophage Glial cell B cell Pericyte Sensory neuron of dorsal root ganglion Fibroblast

Dorsal root ganglion

15-month-old stage 8-week-old stage

Peripheral neuropathy Normal

Female Male

10x 3' v2 10x 3' v3 10x multiome Smart-seq2 Smart-seq3 Indrop

Nucleus Cell

Homo sapiens

Adipocyte of epicardial fat of left ventricle Cardiac endothelial cell Mast cell Lymphoid lineage restricted progenitor cell Immature innate lymphoid cell Neuronal receptor cell Pericyte Cardiac muscle myoblast Smooth muscle myoblast Fibroblast of cardiac tissue

Heart left ventricle

66-year-old stage 43-year-old stage 61-year-old stage 52-year-old stage 44-year-old stage 38-year-old stage 55-year-old stage 51-year-old stage 57-year-old stage 74-year-old stage 58-year-old stage 59-year-old stage 64-year-old stage 60-year-old stage 63-year-old stage 47-year-old stage 40-year-old stage

Normal Myocardial infarction

Female Male

10x 3' v3

Nucleus

Homo sapiens

Germinal center b cell Group 3 innate lymphoid cell, human Dendritic cell, human Granzyme k-associated cd8 t cell Gamma-delta t cell Mast cell Plasmacytoid dendritic cell, human Cd14-positive, cd16-low monocyte Naive thymus-derived cd8-positive, alpha-beta t cell Marginal zone b cell of lymph node Double negative memory b cell Macrophage Cd4-positive, cd25-positive, alpha-beta regulatory t cell Lymph node macrophage Cd16-positive, cd56-dim natural killer cell, human Plasma cell Naive regulatory t cell T follicular helper cell Cd14-low, cd16-positive monocyte Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Transitional stage b cell Naive b cell Fibroblast Class switched memory b cell Cd16-negative, cd56-bright natural killer cell, human Cd4-positive, alpha-beta memory t cell, cd45ro-positive

Axillary lymph node

21-year-old stage 34-year-old stage 38-year-old stage 50-year-old stage 24-year-old stage 35-year-old stage 32-year-old stage 41-year-old stage 22-year-old stage 19-year-old stage 27-year-old stage 40-year-old stage

Normal

Female Male

10x 5' v2

Cell