Homo sapiens

Oligodendrocyte precursor cell Oligodendrocyte Vascular leptomeningeal cell Pvalb chandelier gabaergic interneuron Lamp5 gabaergic interneuron Microglial cell Vip gabaergic interneuron L2/3-6 intratelencephalic projecting glutamatergic neuron L5 extratelencephalic projecting glutamatergic cortical neuron L6b glutamatergic cortical neuron Pvalb gabaergic interneuron Near-projecting glutamatergic cortical neuron Cerebral cortex endothelial cell Caudal ganglionic eminence derived interneuron Sncg gabaergic interneuron Astrocyte of the cerebral cortex Sst gabaergic interneuron Corticothalamic-projecting glutamatergic cortical neuron

Middle temporal gyrus

42-year-old stage 43-year-old stage 29-year-old stage 50-year-old stage Adult stage 60-year-old stage

Normal

Female Male

Smart-seq v4 10x 3' v3

Nucleus

Homo sapiens

Hepatocyte Intestine goblet cell Endothelial cell Mature nk t cell Basal cell Peripheral nervous system neuron Epithelial cell Enteroendocrine cell Glial cell Intestinal epithelial cell Mesenchymal cell Mesothelial cell Ciliated epithelial cell B cell Pericyte Myeloid leukocyte Epithelial cell of lung Erythroblast Chondrocyte

Lung Stomach Duodenum Pyloric antrum Small intestine Epithelium of trachea Ileum Liver Colon Body of stomach Esophagus Jejunum

19th week post-fertilization stage 21st week post-fertilization stage 13th week post-fertilization stage 15th week post-fertilization stage 17th week post-fertilization stage 12th week post-fertilization stage 9th week post-fertilization stage Embryonic stage 18th week post-fertilization stage 11th week post-fertilization stage

Normal

Female Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Astrocyte

Subthalamic nucleus Medial geniculate body Brodmann (1909) area 25 Lateral amygdaloid nucleus Anterior nuclear group Corticomedial nuclear complex Pontine reticular formation Temporal fusiform gyrus Brodmann (1909) area 19 Centromedian nucleus of thalamus Intralaminar nuclear group Medial dorsal nucleus of thalamus Parabrachial nucleus Primary visual cortex Claustrum of brain Inferior temporal gyrus Rostral ca3 Nucleus of medulla oblongata Red nucleus Subicular complex Pontine tegmentum Pontine nuclear group Globus pallidus Midbrain nucleus Piriform cortex Hippocampal field Lateral nuclear group of thalamus Brodmann (1909) area 13 Middle temporal gyrus Central amygdaloid nucleus Ventral anterior nucleus of thalamus Septal nuclear complex Brodmann (1909) area 35 Cerebellar nuclear complex Median tuberal portion Extrastriate cortex Epithalamus Dorsal tegmental nucleus Brodmann (1909) area 38 Lateral entorhinal cortex Supraoptic nucleus Brodmann (1909) area 32 Medial entorhinal cortex Midbrain tectum Inferior olivary complex Brodmann (1909) area 40 Inferior frontal gyrus Substantia nigra Agranular insular cortex Brodmann (1909) area 43 Nucleus accumbens Cerebrocerebellum Gray matter of midbrain Substantia innominata Anterior cortical amygdaloid nucleus Primary somatosensory cortex Brodmann (1909) area 14 Anterior olfactory nucleus Accessory basal amygdaloid nucleus Pulvinar nucleus Primary auditory cortex Hypothalamus Brodmann (1909) area 23 Primary motor cortex Brodmann (1909) area 24 Brodmann (1909) area 46 Medial zone of hypothalamus Posterior parahippocampal gyrus Basolateral amygdaloid nuclear complex Perirhinal cortex Granular insular cortex Cerebellar vermis Spinal cord Retrosplenial region Cuneus cortex Dentate gyrus of hippocampal formation Lateral posterior nucleus of thalamus Mammillary body Superior temporal gyrus Body of caudate nucleus Uncal ca1 Bed nucleus of stria terminalis Superior parietal cortex Lateral geniculate body Putamen Pretectal region Nucleus of dorsal thalamus Preoptic area

42-year-old stage 29-year-old stage 50-year-old stage 60-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Stem cell Paneth cell Epithelial cell Goblet cell Tuft cell Type ec enteroendocrine cell Enterocyte Type l enteroendocrine cell

Ileal epithelium Small intestine Ileum Ileum lamina propria Lamina propria of small intestine Epithelium of small intestine

Sixth decade stage Fifth decade stage Third decade stage Seventh decade stage Fourth decade stage Eighth decade stage

Normal Crohn disease

Female Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Oligodendrocyte precursor cell Oligodendrocyte Vascular leptomeningeal cell Pvalb chandelier gabaergic interneuron Lamp5 gabaergic interneuron Microglial cell Vip gabaergic interneuron L2/3-6 intratelencephalic projecting glutamatergic neuron L5 extratelencephalic projecting glutamatergic cortical neuron L6b glutamatergic cortical neuron Pvalb gabaergic interneuron Near-projecting glutamatergic cortical neuron Cerebral cortex endothelial cell Caudal ganglionic eminence derived interneuron Sncg gabaergic interneuron Astrocyte of the cerebral cortex Sst gabaergic interneuron Corticothalamic-projecting glutamatergic cortical neuron

Primary somatosensory cortex

42-year-old stage 43-year-old stage 29-year-old stage 50-year-old stage 60-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

T cell Natural killer cell Mast cell Cd8-positive, alpha-beta t cell Leukocyte Plasmacytoid dendritic cell Macrophage Innate lymphoid cell Regulatory t cell Plasma cell Cd4-positive, alpha-beta t cell B cell Naive t cell Monocyte Conventional dendritic cell Dendritic cell

Transverse colon epithelium Ascending colon Colonic epithelium Sigmoid colon lamina propria Lamina propria of mucosa of colon Transverse colon lamina propria Colon Caecum lamina propria Sigmoid colon epithelium Caecum epithelium Descending colon Sigmoid colon

Sixth decade stage Fifth decade stage Third decade stage Seventh decade stage Adult stage Fourth decade stage Eighth decade stage

Normal Crohn disease

Female Male

10x 3' v1 10x 3' v3 10x 3' v2

Cell

Homo sapiens

Natural killer cell Endothelial cell of lymphatic vessel Cytotoxic t cell Mast cell Cd8-positive, alpha-beta memory t cell, cd45ro-positive Basal cell Schwann cell Smooth muscle cell Plasmacytoid dendritic cell Endothelial cell of vascular tree Basal cell of epidermis Macrophage Innate lymphoid cell Regulatory t cell Helper t cell Plasma cell Cell of skeletal muscle Pericyte Melanocyte of skin Monocyte Suprabasal keratinocyte Conventional dendritic cell Fibroblast Dendritic cell Chondrocyte

Skin of abdomen Skin of cheek Skin of external ear Skin of forehead Arm skin Skin of temple Nose skin

66-year-old stage 69-year-old stage 91-year-old stage 85-year-old stage 53-year-old stage 54-year-old stage 39-year-old stage 56-year-old stage 90-year-old stage 80-year-old stage 51-year-old stage 81-year-old stage 24-year-old stage 23-year-old stage 59-year-old stage 25-year-old stage 77-year-old stage 62-year-old stage 86-year-old stage 70-year-old stage 78-year-old stage 63-year-old stage 73-year-old stage 27-year-old stage

Normal Basal cell carcinoma

Female Male

10x 3' v1 10x 3' v3 10x 3' v2

Cell

Homo sapiens

Medium spiny neuron

Brodmann (1909) area 25 Lateral amygdaloid nucleus Anterior nuclear group Corticomedial nuclear complex Claustrum of brain Globus pallidus Piriform cortex Hippocampal field Lateral nuclear group of thalamus Brodmann (1909) area 13 Middle temporal gyrus Central amygdaloid nucleus Ventral anterior nucleus of thalamus Septal nuclear complex Cerebellar nuclear complex Brodmann (1909) area 38 Supraoptic nucleus Medial entorhinal cortex Substantia nigra Agranular insular cortex Nucleus accumbens Gray matter of midbrain Substantia innominata Anterior cortical amygdaloid nucleus Anterior olfactory nucleus Accessory basal amygdaloid nucleus Pulvinar nucleus Primary auditory cortex Hypothalamus Primary motor cortex Brodmann (1909) area 24 Basolateral amygdaloid nuclear complex Perirhinal cortex Granular insular cortex Dentate gyrus of hippocampal formation Lateral posterior nucleus of thalamus Mammillary body Superior temporal gyrus Body of caudate nucleus Uncal ca1 Bed nucleus of stria terminalis Lateral geniculate body Putamen Pretectal region Preoptic area

42-year-old stage 29-year-old stage 50-year-old stage 60-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Central memory cd4-positive, alpha-beta t cell T cell Cd1c-positive myeloid dendritic cell Mature gamma-delta t cell Natural killer cell Erythrocyte Platelet Cd141-positive myeloid dendritic cell Effector memory cd4-positive, alpha-beta t cell Intermediate monocyte Cd8aa(i) thymocyte Plasmacytoid dendritic cell Cd8-positive, alpha-beta memory t cell Be cell Memory regulatory t cell Naive thymus-derived cd8-positive, alpha-beta t cell Memory b cell Axl+ dendritic cell, human Cd16-positive, cd56-dim natural killer cell, human Innate lymphoid cell Common myeloid progenitor Plasma cell Naive regulatory t cell Memory ccr4-positive regulatory t cell Cd14-low, cd16-positive monocyte Cd4-positive, alpha-beta memory t cell Naive thymus-derived cd4-positive, alpha-beta t cell Common lymphoid progenitor Mucosal invariant t cell Cd8-positive, alpha-beta regulatory t cell Transitional stage b cell Cd14-positive, cd16-negative classical monocyte Central memory cd8-positive, alpha-beta t cell Naive b cell Double negative thymocyte Cd16-negative, cd56-bright natural killer cell, human Effector memory cd8-positive, alpha-beta t cell

Blood

37-year-old stage 53-year-old stage 61-year-old stage 52-year-old stage 38-year-old stage 50-year-old stage 51-year-old stage 59-year-old stage 41-year-old stage

Rheumatoid arthritis Cytomegalovirus infection

Female Male

10x 3' v3

Cell

Callithrix jacchus

T cell L2/3 intratelencephalic projecting glutamatergic neuron Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Vascular leptomeningeal cell Erythroid lineage cell Smooth muscle cell Inhibitory interneuron Pvalb chandelier gabaergic interneuron Lamp5 gabaergic interneuron Macrophage L6 intratelencephalic projecting glutamatergic neuron of the primary motor cortex Microglial cell Vip gabaergic interneuron Myeloid cell L5 extratelencephalic projecting glutamatergic cortical neuron L6b glutamatergic cortical neuron L6 corticothalamic-projecting glutamatergic cortical neuron Pvalb gabaergic interneuron B cell Pericyte Astrocyte L5/6 near-projecting glutamatergic neuron of the primary motor cortex Sst gabaergic interneuron

Dorsolateral prefrontal cortex

Prime adult stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Erythrocyte Early lymphoid progenitor Macrophage Promonocyte Hematopoietic stem cell Common myeloid progenitor Hematopoietic multipotent progenitor cell Megakaryocyte Granulocyte monocyte progenitor cell Promyelocyte Megakaryocyte-erythroid progenitor cell Fraction a pre-pro b cell Vascular associated smooth muscle cell Double negative thymocyte

Skin of body Thymus Yolk sac Liver Gut wall Kidney Spleen Bone marrow Mesenteric lymph node

Carnegie stage 19 13th week post-fertilization stage Carnegie stage 13 14th week post-fertilization stage 15th week post-fertilization stage 17th week post-fertilization stage 12th week post-fertilization stage 16th week post-fertilization stage Carnegie stage 20 9th week post-fertilization stage Carnegie stage 23 10th week post-fertilization stage Carnegie stage 22 11th week post-fertilization stage

Normal

Female Male

10x 5' v1 10x 3' v2

Cell

Callithrix jacchus

Astrocyte

Brodmann (1909) area 25 Lateral amygdaloid nucleus Dorsolateral prefrontal cortex Thalamic complex Parietal cortex Medial dorsal nucleus of thalamus Primary visual cortex Ventral lateral nucleus of thalamus Brainstem Temporal cortex Lateral nuclear group of thalamus Caudate nucleus Prefrontal cortex Anterior hypothalamic region Temporal lobe Central amygdaloid nucleus Cerebellum lobule Nucleus accumbens Ventral thalamus Basal forebrain Primary somatosensory cortex Primary auditory cortex Brodmann (1909) area 23 Primary motor cortex Dorsal thalamus Hippocampal formation Secondary visual cortex Posterior hypothalamic region Lateral geniculate body Putamen Medial amygdaloid nucleus Amygdala

Late adult stage Prime adult stage

Normal

Female Male

10x 3' v3

Nucleus