Homo sapiens

L2/3-6 intratelencephalic projecting glutamatergic neuron

Middle temporal gyrus

Adult stage 78-year-old stage 80 year-old and over stage 72-year-old stage 83-year-old stage 85-year-old stage 81-year-old stage 82-year-old stage 86-year-old stage 70-year-old stage 75-year-old stage 50-year-old stage 89-year-old stage 68-year-old stage 29-year-old stage 77-year-old stage 69-year-old stage 88-year-old stage 87-year-old stage 60-year-old stage 80-year-old stage 84-year-old stage 43-year-old stage 65-year-old stage

Dementia Normal

Female Male

10x multiome 10x 3' v3

Nucleus

Homo sapiens

Neuron

Intralaminar nuclear group Primary motor cortex Cerebellar nuclear complex Dentate gyrus of hippocampal formation Ventral anterior nucleus of thalamus Subthalamic nucleus Globus pallidus Midbrain tectum Lateral nuclear group of thalamus Epithalamus Medial dorsal nucleus of thalamus Medial zone of hypothalamus Lateral geniculate body Nucleus of medulla oblongata Red nucleus Corticomedial nuclear complex Nucleus of dorsal thalamus Pontine reticular formation Midbrain nucleus Pontine tegmentum Accessory basal amygdaloid nucleus Anterior olfactory nucleus Hypothalamus Centromedian nucleus of thalamus Substantia nigra Septal nuclear complex Basolateral amygdaloid nuclear complex Spinal cord Preoptic area Parabrachial nucleus Pulvinar nucleus Lateral amygdaloid nucleus Pontine nuclear group Median tuberal portion Dorsal tegmental nucleus Substantia innominata Bed nucleus of stria terminalis Medial geniculate body Inferior olivary complex Gray matter of midbrain Mammillary body Anterior nuclear group Central amygdaloid nucleus Pretectal region Lateral posterior nucleus of thalamus

42-year-old stage 50-year-old stage 29-year-old stage 60-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Mus musculus

Myelinating schwann cell Melanocyte Olfactory ensheathing cell Schwann cell precursor Perineuronal satellite cell

Embryo

Theiler stage 26 Theiler stage 18 Theiler stage 13 Theiler stage 24 Theiler stage 15 Theiler stage 14 Theiler stage 12 Theiler stage 23 Theiler stage 20 Theiler stage 22 Theiler stage 16 Theiler stage 21 Theiler stage 25 Theiler stage 27 Theiler stage 17 Theiler stage 19

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Myeloid cell Oligodendrocyte Neoplastic cell Pericyte Lymphocyte Endothelial cell

Diffuse glioma, h3 g34 mutant Glioblastoma Oligodendroglioma, idh-mutant and 1p/19q-codeleted, grade 3

Single-cell rna sequencing

Macaca fascicularis

Fibroblast Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Interneuron Ependymal cell Endothelial stalk cell Microglial cell Neuron

Normal

10x 3' v3

Homo sapiens

Fibroblast Primitive red blood cell Muscle fibroblast Schwann cell Common myeloid progenitor Fibroblast of dermis Mast cell Articular chondrocyte Vein endothelial cell Early lymphoid progenitor Smooth muscle cell Peridermal cell Melanocyte Chondroblast B cell Skeletal muscle myoblast Mesenchymal cell Chondrocyte Megakaryocyte Pericyte Muscle cell Endothelial cell of lymphatic vessel Tendon cell Natural killer cell Macrophage Hypertrophic chondrocyte Reticulocyte Myelocyte Schwann cell precursor Erythrocyte Osteoblast Conventional dendritic cell Monocyte Basal cell Myoblast Neuron Connective tissue cell Endothelial cell of artery

Forelimb Hindlimb

Carnegie stage 19 Carnegie stage 18 10th week post-fertilization stage Carnegie stage 23 Carnegie stage 15 Carnegie stage 17 9th week post-fertilization stage Carnegie stage 16

Normal

Female Male

10x 5' v1 10x 3' v2

Cell

Homo sapiens

Lung pericyte Pancreatic acinar cell Mast cell Dendritic cell, human Intermediate monocyte Smooth muscle cell Neutrophil Stromal cell Plasma cell B cell Gamma-delta t cell Regulatory t cell Fibroblast of lung Pulmonary capillary endothelial cell Epithelial cell of lung Club cell Non-classical monocyte Plasmacytoid dendritic cell, human Pulmonary alveolar type 2 cell Cd8-positive, alpha-beta t cell Pulmonary alveolar type 1 cell Lung macrophage Natural killer cell Lung endothelial cell Erythrocyte Mature nk t cell Basal cell Mesothelial cell Cd4-positive, alpha-beta t cell Lung multiciliated epithelial cell Myeloid dendritic cell, human

Lower lobe of left lung

57-year-old stage

Covid-19

Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Regular ventricular cardiac myocyte

Heart left ventricle Apex of heart Interventricular septum Heart right ventricle

Sixth decade stage Eighth decade stage Fifth decade stage Seventh decade stage

Normal

Female Male

10x 3' v3 10x 3' v2

Nucleus

Homo sapiens

Cd16-positive, cd56-dim natural killer cell, human Granulocyte Intermediate monocyte Non-classical monocyte Plasmacytoid dendritic cell Cd16-negative, cd56-bright natural killer cell, human Natural killer cell Platelet Conventional dendritic cell Classical monocyte

Blood

54-year-old stage 35-year-old stage 78-year-old stage 73-year-old stage 74-year-old stage 53-year-old stage 61-year-old stage 57-year-old stage 49-year-old stage 52-year-old stage 70-year-old stage 40-year-old stage 45-year-old stage 59-year-old stage 30-year-old stage 55-year-old stage 58-year-old stage 50-year-old stage 51-year-old stage 79-year-old stage 66-year-old stage 77-year-old stage 32-year-old stage 76-year-old stage 63-year-old stage 60-year-old stage 84-year-old stage 37-year-old stage 24-year-old stage 67-year-old stage 71-year-old stage 47-year-old stage

Covid-19 Normal

Female Male

10x 5' v1

Cell

Homo sapiens

Cortical interneuron Neural progenitor cell Astrocyte Inhibitory interneuron Oligodendrocyte precursor cell Oligodendrocyte Brain vascular cell Ependymal cell Glutamatergic neuron Microglial cell

Ganglionic eminence Entorhinal cortex

27-year-old stage 21st week post-fertilization stage 13-year-old stage Newborn stage (0-28 days) 1-month-old stage 3-year-old stage 2-month-old stage 2-year-old stage 50-year-old stage 51-year-old stage 79-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Innate lymphoid cell Plasmablast Effector memory cd4-positive, alpha-beta t cell Mucosal invariant t cell Germinal center b cell Cd16-positive, cd56-dim natural killer cell, human Mast cell Effector memory cd8-positive, alpha-beta t cell Dendritic cell, human Central memory cd4-positive, alpha-beta t cell Stromal cell Memory b cell Plasma cell B cell Regulatory t cell Activated cd4-positive, alpha-beta t cell Non-classical monocyte Plasmacytoid dendritic cell Endothelial cell of lymphatic vessel Cd16-negative, cd56-bright natural killer cell, human Central memory cd8-positive, alpha-beta t cell Macrophage Conventional dendritic cell Classical monocyte T cell Naive b cell T follicular helper cell Endothelial cell

Bronchopulmonary lymph node Mesenteric lymph node Thoracic lymph node Lymph node

Third decade stage Sixth decade stage 52-year-old stage Eighth decade stage Fifth decade stage 65-year-old stage Seventh decade stage

Normal

Female Male

10x 3' v3 10x 5' v2 10x 5' v1 10x 3' v2

Cell

Homo sapiens

Cytotoxic t cell Cd14-low, cd16-positive monocyte Cd14-positive monocyte Dendritic cell B cell Megakaryocyte Plasmacytoid dendritic cell Natural killer cell Cd4-positive, alpha-beta t cell

Blood

42-year-old stage

Normal

Male

10x gene expression flex Bd rhapsody whole transcriptome analysis Scalebio single cell rna sequencing 10x 3' v3 Parse evercode whole transcriptome v2 10x 5' v2 Hive clx single-cell rnaseq solution Asteria scrna-seq kit Particle-templated instant partition sequencing

Cell