Homo sapiens

Stromal cell Natural killer cell Endothelial cell of lymphatic vessel Secretory cell Endothelial cell Mast cell Smooth muscle cell Macrophage Ciliated epithelial cell B cell Pericyte

Ampulla of fallopian tube Fimbria of fallopian tube Isthmus of fallopian tube

65-year-old stage 61-year-old stage 55-year-old stage 62-year-old stage

Normal

Female

10x scatac-seq

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Cerebellar inhibitory gabaergic interneuron Microglial cell B cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Posterior parahippocampal gyrus

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus Tupaia belangeri

Neuron

Normal

10x 3' v3

Homo sapiens

Lung interstitial macrophage Plasmacytoid dendritic cell Lung macrophage Metallothionein-positive alveolar macrophage Alveolar macrophage Cd14-positive monocyte Monocyte Conventional dendritic cell Dendritic cell

Bronchus Lung Trachea Upper lobe of left lung Lower lobe of left lung

66-year-old stage 75-year-old stage Sixth decade stage 29-year-old stage 35-year-old stage Seventh decade stage 58-year-old stage 59-year-old stage 64-year-old stage

Normal

Female Male

10x 5' transcription profiling 10x 3' v3 10x 3' v2

Cell Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Choroid plexus epithelial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Superior temporal gyrus

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Podocyte Endothelial cell Kidney loop of henle thin descending limb epithelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney connecting tubule epithelial cell Kidney loop of henle thick ascending limb epithelial cell Kidney loop of henle epithelial cell Mesangial cell Pericyte Kidney proximal convoluted tubule epithelial cell

Cortex of kidney

62-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Early lymphoid progenitor Promonocyte Hematopoietic stem cell Common myeloid progenitor Hematopoietic multipotent progenitor cell Megakaryocyte Granulocyte monocyte progenitor cell Promyelocyte Megakaryocyte-erythroid progenitor cell Fraction a pre-pro b cell Double negative thymocyte

Skin of body Thymus Yolk sac Liver Gut wall Kidney Spleen Bone marrow Mesenteric lymph node

Carnegie stage 19 13th week post-fertilization stage Carnegie stage 13 14th week post-fertilization stage 15th week post-fertilization stage 17th week post-fertilization stage 12th week post-fertilization stage 16th week post-fertilization stage Carnegie stage 20 9th week post-fertilization stage Carnegie stage 23 10th week post-fertilization stage Carnegie stage 22 11th week post-fertilization stage

Normal

Female Male

10x 5' v1 10x 3' v2

Cell

Homo sapiens

Neutrophil Podocyte Renal principal cell Natural killer cell Renal beta-intercalated cell Glomerular endothelial cell Mature nk t cell Kidney interstitial fibroblast Mast cell Cd8-positive, alpha-beta t cell Kidney resident macrophage Capillary endothelial cell Classical monocyte Epithelial cell of proximal tubule Plasmacytoid dendritic cell Renal intercalated cell Vasa recta descending limb cell Renal alpha-intercalated cell Kidney connecting tubule epithelial cell Urothelial cell Kidney loop of henle thick ascending limb epithelial cell Vasa recta ascending limb cell Cd4-positive, alpha-beta t cell Kidney epithelial cell B cell Progenitor cell Myofibroblast cell Dendritic cell Non-classical monocyte

Renal medulla Renal pelvis Kidney blood vessel Kidney Cortex of kidney

53-year-old stage 2-year-old stage 44-year-old stage 12-year-old stage 72-year-old stage 49-year-old stage 67-year-old stage 64-year-old stage 19-month-old stage 70-year-old stage 63-year-old stage 4-year-old stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Neutrophil Renal principal cell Natural killer cell Renal beta-intercalated cell Glomerular endothelial cell Mast cell Cd8-positive, alpha-beta t cell Peritubular capillary endothelial cell Epithelial cell of proximal tubule Plasmacytoid dendritic cell Renal intercalated cell Vasa recta descending limb cell Renal alpha-intercalated cell Lymphocyte Kidney connecting tubule epithelial cell Urothelial cell Epithelial cell of glomerular capsule Kidney loop of henle thick ascending limb epithelial cell Vasa recta ascending limb cell Cd4-positive, alpha-beta t cell Kidney epithelial cell B cell Progenitor cell Myofibroblast cell Fibroblast Mononuclear phagocyte

Renal medulla Renal pelvis Kidney blood vessel Kidney Cortex of kidney

53-year-old stage 2-year-old stage 44-year-old stage 12-year-old stage 72-year-old stage 49-year-old stage 67-year-old stage 64-year-old stage 19-month-old stage 70-year-old stage 63-year-old stage 4-year-old stage

Normal

Female Male

10x 3' v2

Cell

Mus musculus

Granulocyte Natural killer cell Hematopoietic precursor cell Erythroid progenitor cell, mammalian Late pro-b cell Precursor b cell Macrophage Promonocyte Plasma cell Granulocytopoietic cell Naive t cell Immature b cell Megakaryocyte-erythroid progenitor cell Monocyte Naive b cell Erythroblast Proerythroblast Basophil

Bone marrow

4-week-old stage 3-month-old stage 20-month-old stage and over 18-month-old stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Cd8-positive, alpha-beta t cell Exhausted t cell Cd4-positive helper t cell Regulatory t cell Myeloid cell Plasma cell Malignant cell B cell

Lung

61-year-old stage

Renal cell carcinoma

Male

Slide-seqv2

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Dentate gyrus granule cell Cerebellar granule cell Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Thalamic excitatory neuron Choroid plexus epithelial cell B cell Pericyte Cerebral cortex neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron Hippocampal ca4 neuron

Dentate gyrus of hippocampal formation

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus