Drosophila melanogaster

Neuron Fat cell Hemocyte Epithelial cell Germline cell Adult alary muscle cell Muscle cell Adult neuron Sensory neuron Oenocyte Adult oenocyte

Fat body

Day 3 of adulthood

Normal

Female Male

10x 3' v3

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2

Homo sapiens

Natural killer cell Erythroid progenitor cell Gamma-delta t cell Pro-b cell Erythroid lineage cell Classical monocyte Pre-natural killer cell Plasmacytoid dendritic cell Precursor b cell Lymphoid lineage restricted progenitor cell Basophil mast progenitor cell Megakaryocyte progenitor cell Common dendritic progenitor Cd8-positive, alpha-beta memory t cell Cd4-positive, alpha-beta cytotoxic t cell Naive thymus-derived cd8-positive, alpha-beta t cell Unswitched memory b cell Memory b cell Cd16-positive, cd56-dim natural killer cell, human Myelocyte Plasma cell Late promyelocyte Mesenchymal cell Hematopoietic multipotent progenitor cell Cd4-positive, alpha-beta memory t cell Progenitor cell Naive thymus-derived cd4-positive, alpha-beta t cell Early promyelocyte Immature b cell Megakaryocyte-erythroid progenitor cell Fraction a pre-pro b cell Central memory cd8-positive, alpha-beta t cell Naive b cell Conventional dendritic cell Class switched memory b cell Cd16-negative, cd56-bright natural killer cell, human Effector memory cd8-positive, alpha-beta t cell Non-classical monocyte

Bone marrow

66-year-old stage 71-year-old stage 61-year-old stage 54-year-old stage 44-year-old stage 56-year-old stage 55-year-old stage 51-year-old stage 32-year-old stage 45-year-old stage 62-year-old stage 70-year-old stage 46-year-old stage 78-year-old stage

Acute myeloid leukemia Acute promyelocytic leukemia

Female Male

Bd rhapsody targeted mrna

Cell

Homo sapiens

Neutrophil Blood vessel endothelial cell Natural killer cell Endothelial cell of lymphatic vessel Cd8-positive, alpha-beta t cell Adult skeletal muscle myoblast Cd4-positive helper t cell Ionocyte Myoepithelial cell Macrophage Mural cell Keratinocyte Acinar cell of salivary gland Plasma cell Salivary gland glandular cell B cell Fibroblast Dendritic cell

Minor salivary gland

34-year-old stage 26-year-old stage 53-year-old stage 48-year-old stage 39-year-old stage 56-year-old stage 28-year-old stage 24-year-old stage 40-year-old stage

Normal

Female Male

10x 3' v3

Cell

Drosophila melanogaster

Tracheolar cell Somatic precursor cell Polar follicle cell Dorsal appendage forming follicle cell Posterior terminal follicle cell Stretch follicle cell Epithelial cell Germline cell Muscle cell Escort cell Adult tracheocyte Ovarian sheath muscle cell Main body follicle cell Stalk follicle cell Female germline cell

Ovary

Day 5 of adulthood

Normal

Female

10x 3' v3

Mus musculus

Oligodendrocyte precursor cell Committed oligodendrocyte precursor

Embryo

Theiler stage 22 Theiler stage 27 Theiler stage 24 Theiler stage 25 Theiler stage 19 Theiler stage 26 Theiler stage 20 Theiler stage 21 Theiler stage 23

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Thalamic excitatory neuron Choroid plexus epithelial cell B cell Pericyte Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Anterior nuclear group

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Hepatic stellate cell Hepatic portal fibroblast Vascular associated smooth muscle cell

Left lobe of liver Right lobe of liver Caudate lobe of liver Liver

37-year-old stage 21-year-old stage 65-year-old stage 26-year-old stage Sixth decade stage 71-year-old stage 53-year-old stage 61-year-old stage 52-year-old stage 54-year-old stage 48-year-old stage 39-year-old stage 44-year-old stage 56-year-old stage Fifth decade stage 55-year-old stage 50-year-old stage 80-year-old stage 51-year-old stage 57-year-old stage 72-year-old stage Third decade stage 49-year-old stage Seventh decade stage 58-year-old stage 59-year-old stage Adult stage 67-year-old stage Late adult stage 32-year-old stage 33-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 41-year-old stage 70-year-old stage 60-year-old stage Fourth decade stage Eighth decade stage Prime adult stage Ninth decade stage Young adult stage

Normal

Female Male

10x 5' v1 10x 3' v3 10x 3' v2

Nucleus Cell

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Anterior olfactory nucleus

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Autosomal dominant polycystic kidney disease

Male

Slide-seqv2

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Thalamic excitatory neuron Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron

Accessory basal amygdaloid nucleus

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Brodmann (1909) area 46

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus