Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Dentate gyrus granule cell Cerebellar granule cell Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Ependymal cell Microglial cell Choroid plexus epithelial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron Hippocampal ca4 neuron

Hippocampal field

42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Malignant cell

Pancreatic ductal adenocarcinoma

Seq-well s3

Homo sapiens

Vip gabaergic interneuron

Neocortex

Adult stage

Normal

Female Male

10x 3' v3

Cell

Homo sapiens

Luminal cell of prostate epithelium Prostate gland microvascular endothelial cell Secretory cell Neuroendocrine cell Leukocyte Fibroblast of connective tissue of prostate Smooth muscle cell of prostate Epithelial cell of urethra Basal cell of prostate epithelium

Transition zone of prostate Peripheral zone of prostate

29-year-old stage 31-year-old stage

Normal

Male

10x 3' v2

Cell

Homo sapiens

Blood vessel endothelial cell Endothelial cell of lymphatic vessel Smooth muscle cell Pericyte Myofibroblast cell Fibroblast

Ampulla of fallopian tube Fimbria of fallopian tube Fallopian tube Isthmus of fallopian tube

52-year-old stage 30-year-old stage 46-year-old stage 31-year-old stage

Normal

Female

10x 3' v3

Cell

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Autosomal dominant polycystic kidney disease

Male

Slide-seqv2

Homo sapiens

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Cortex of kidney

72-year-old stage

Normal

Male

Slide-seqv2

Drosophila melanogaster

Adult somatic muscle cell Hemocyte Maxillary palp olfactory receptor neuron Epithelial cell Tormogen cell Labral sense organ mechanosensory neuron Gustatory receptor neuron of the labellum

Proboscis Maxillary palp

Day 5 of adulthood

Normal

Female Male

10x 3' v3

Mus musculus

Erythrocyte Primary neuron (sensu teleostei) Endothelial cell Schwann cell Macrophage Innate lymphoid cell Myeloid cell Perineuronal satellite cell Pericyte Fibroblast

Lesion of sciatic nerve Normal

Mixed sex

10x 3' v3

Homo sapiens

T cell Endothelial cell Mast cell Myeloid cell B cell Fibroblast Oral mucosa squamous cell

Oral cavity

65-year-old stage 38-year-old stage 55-year-old stage 50-year-old stage 49-year-old stage 62-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 36-year-old stage 76-year-old stage

Oral cavity squamous cell carcinoma

Female Male

10x 3' v3

Cell

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Hippocampal ca1-3 neuron Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Cerebellar inhibitory gabaergic interneuron Ependymal cell Microglial cell Choroid plexus epithelial cell Bergmann glial cell B cell Pericyte Cerebral cortex neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast Medium spiny neuron Mammillary body neuron

Mammillary body

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Cuneus cortex

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus