Homo sapiens

Cd8-positive, alpha-beta t cell Exhausted t cell Cd4-positive helper t cell Regulatory t cell Myeloid cell Plasma cell Malignant cell T follicular helper cell B cell

Lymph node

Adult stage

Metastatic melanoma

Slide-seqv2

Drosophila melanogaster

Sensory neuron of campaniform sensillum Indirect flight muscle cell Fat cell Glial cell Hemocyte Chordotonal neuron Embryonic/larval crystal cell Mechanosensory neuron Oenocyte Tendon cell

Body wall

Day 5 of adulthood

Normal

Female Male

10x 3' v3

Homo sapiens

T cell Blood vessel endothelial cell Blood vessel smooth muscle cell Macrophage Malignant cell Fibroblast

Axilla

27-year-old stage

Breast cancer

Female

10x 3' v3

Nucleus

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Lamp5 lhx6 neuron Eccentric medium spiny neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Cerebellar inhibitory gabaergic interneuron Ependymal cell Microglial cell Thalamic excitatory neuron Pericyte Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Medial dorsal nucleus of thalamus

42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2

Homo sapiens

Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Kidney proximal convoluted tubule epithelial cell

Renal medulla

50-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell

Renal medulla

56-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Luminal cell of prostate epithelium Prostate neuroendocrine cell Epithelial cell of prostate Ciliated epithelial cell Basal cell of prostate epithelium Club-like cell of the urethral epithelium

Prostate gland

69-year-old stage 75-year-old stage 71-year-old stage 61-year-old stage 54-year-old stage 68-year-old stage 72-year-old stage 59-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 63-year-old stage

Prostatic acinar adenocarcinoma

Male

10x 3' v3

Cell

Homo sapiens

Blood vessel endothelial cell Endothelial cell of artery Vein endothelial cell Pulmonary artery endothelial cell

Bronchus Lung Trachea Upper lobe of left lung Lower lobe of left lung

66-year-old stage 75-year-old stage Sixth decade stage 29-year-old stage 35-year-old stage Seventh decade stage 58-year-old stage 59-year-old stage 64-year-old stage

Normal

Female Male

10x 5' transcription profiling 10x 3' v3 10x 3' v2

Cell Nucleus

Homo sapiens

Thymic fibroblast type 1 Thymic fibroblast type 2 Fibroblast

Thymus

13th week post-fertilization stage 2-year-old stage 3-month-old stage 14th week post-fertilization stage 15th week post-fertilization stage 20th week post-fertilization stage 17th week post-fertilization stage 12th week post-fertilization stage 6-month-old stage 18th week post-fertilization stage 10-month-old stage Newborn stage (0-28 days) 24th week post-fertilization stage

Normal

Female Male

10x 5' v2 10x 5' v1 10x 3' v3 10x 3' v2

Cell

Homo sapiens

Near-projecting glutamatergic cortical neuron

Middle temporal gyrus

69-year-old stage 75-year-old stage 65-year-old stage 89-year-old stage 43-year-old stage 87-year-old stage 85-year-old stage 88-year-old stage 68-year-old stage 29-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 80-year-old stage 72-year-old stage 81-year-old stage 80 year-old and over stage Adult stage 77-year-old stage 86-year-old stage 70-year-old stage 60-year-old stage 78-year-old stage 83-year-old stage

Normal Dementia

Female Male

10x multiome 10x 3' v3

Nucleus

Mus musculus

Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Autosomal dominant polycystic kidney disease

Male

Slide-seqv2