Homo sapiens

Glutamatergic neuron

Brodmann (1909) area 9 Brodmann (1909) area 7 Brodmann (1909) area 17

85-year-old stage 80-year-old stage 91-year-old stage 52-year-old stage 68-year-old stage 82-year-old stage 58-year-old stage 73-year-old stage 75-year-old stage 93-year-old stage 86-year-old stage 92-year-old stage 66-year-old stage 61-year-old stage 57-year-old stage 83-year-old stage 89-year-old stage 97-year-old stage 111-year-old stage 70-year-old stage 81-year-old stage 76-year-old stage 67-year-old stage 88-year-old stage 87-year-old stage 62-year-old stage 79-year-old stage 71-year-old stage

Normal Alzheimer disease

Female Male

10x 3' v2 10x 3' v3 Drop-seq

Nucleus

Homo sapiens

Natural killer cell Schwann cell Sebaceous gland cell Neutrophil Naive b cell Cd8-positive, alpha-beta t cell Endothelial cell of lymphatic vessel Regulatory t cell Capillary endothelial cell Mast cell Igg plasma cell Pericyte Macrophage Gamma-delta t cell Dendritic cell T cell Plasmablast Vein endothelial cell Hair follicular keratinocyte Sweat secreting cell Iga plasma cell Skin fibroblast Innate lymphoid cell Smooth muscle cell Endothelial cell of artery Keratinocyte Melanocyte

Normal Atopic eczema

10x 3' v2 10x 3' v3

Macaca mulatta

Oligodendrocyte

Hippocampal formation Cerebrocerebellum Medial dorsal nucleus of thalamus Medulla oblongata Ventral lateral nucleus of thalamus Inferior parietal cortex Cerebellar vermis Brodmann (1909) area 19 Entorhinal cortex Anterior cingulate cortex Primary motor cortex Inferior temporal gyrus Primary visual cortex Corpus callosum Lateral geniculate body Head of caudate nucleus Superior temporal sulcus Primary auditory cortex Primary somatosensory cortex Dorsolateral prefrontal cortex Amygdala Perirhinal cortex Prefrontal cortex Nucleus accumbens Midbrain Superior parietal cortex Pons

Juvenile stage Prime adult stage

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Oligodendrocyte

Optic disc Cranial nerve ii

85-year-old stage 64-year-old stage 50-year-old stage 52-year-old stage 65-year-old stage 68-year-old stage 82-year-old stage 43-year-old stage 63-year-old stage 90 year-old and over stage 15-year-old stage 37-year-old stage 58-year-old stage 75-year-old stage 3-year-old stage 34-year-old stage 42-year-old stage 54-year-old stage 61-year-old stage 11-year-old stage 74-year-old stage 21-year-old stage 53-year-old stage 46-year-old stage 56-year-old stage 77-year-old stage 47-year-old stage 32-year-old stage 45-year-old stage 70-year-old stage 81-year-old stage 76-year-old stage 69-year-old stage 67-year-old stage 60-year-old stage 62-year-old stage 16-year-old stage 10-year-old stage 18-year-old stage 30-year-old stage 72-year-old stage 27-year-old stage 17-year-old stage 71-year-old stage 33-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Mus musculus

Oligodendrocyte Pericyte Leptomeningeal cell Microglial cell Endothelial cell Gabaergic neuron Smooth muscle cell Glutamatergic neuron Astrocyte

Primary motor cortex

Prime adult stage

Normal

Male

Merfish

Homo sapiens

Cd4-positive, cd25-positive, ccr4-positive, alpha-beta regulatory t cell Natural killer cell Mast cell Igg plasma cell Memory b cell Macrophage Cd8-positive, alpha-beta regulatory t cell Naive t cell Cd4-positive helper t cell Conventional dendritic cell Exhausted t cell Cycling t cell Effector memory cd4-positive, alpha-beta t cell Plasmacytoid dendritic cell Cycling macrophage Myeloid dendritic cell T follicular helper cell Effector memory cd8-positive, alpha-beta t cell

Breast

80 year-old and over stage 85-year-old stage 80-year-old stage 64-year-old stage 50-year-old stage 52-year-old stage 65-year-old stage 82-year-old stage 43-year-old stage 41-year-old stage 35-year-old stage Fifth decade stage 63-year-old stage 37-year-old stage 29-year-old stage 58-year-old stage Ninth decade stage 73-year-old stage Adult stage Late adult stage 84-year-old stage 34-year-old stage 42-year-old stage 49-year-old stage 59-year-old stage 54-year-old stage 25-year-old stage 66-year-old stage 61-year-old stage 57-year-old stage Eighth decade stage 83-year-old stage 46-year-old stage 55-year-old stage Seventh decade stage 47-year-old stage 36-year-old stage 32-year-old stage 60-79 year-old stage 45-year-old stage 70-year-old stage 69-year-old stage 67-year-old stage 88-year-old stage 60-year-old stage 62-year-old stage Sixth decade stage 38-year-old stage Prime adult stage 30-year-old stage 79-year-old stage 71-year-old stage Middle aged stage

Estrogen-receptor positive breast cancer Invasive lobular breast carcinoma Triple-negative breast carcinoma Her2 positive breast carcinoma Invasive ductal breast carcinoma Invasive tubular breast carcinoma Breast apocrine carcinoma Metaplastic breast carcinoma Breast cancer Breast mucinous carcinoma Breast carcinoma

Female Male

10x 3' v2 10x 3' v3 10x 5' transcription profiling 10x 5' v2 10x 5' v1

Cell

Homo sapiens

Retinal astrocyte Astrocyte

Optic disc Cranial nerve ii

85-year-old stage 64-year-old stage 50-year-old stage 52-year-old stage 65-year-old stage 68-year-old stage 82-year-old stage 43-year-old stage 63-year-old stage 90 year-old and over stage 15-year-old stage 37-year-old stage 58-year-old stage 75-year-old stage 3-year-old stage 34-year-old stage 42-year-old stage 54-year-old stage 25-year-old stage 61-year-old stage 11-year-old stage 74-year-old stage 21-year-old stage 53-year-old stage 46-year-old stage 56-year-old stage 77-year-old stage 47-year-old stage 32-year-old stage 45-year-old stage 70-year-old stage 81-year-old stage 76-year-old stage 69-year-old stage 67-year-old stage Newborn stage (0-28 days) 60-year-old stage 62-year-old stage 16-year-old stage 10-year-old stage 18-year-old stage 30-year-old stage 72-year-old stage 27-year-old stage 17-year-old stage 71-year-old stage 33-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Naive b cell Memory b cell Precursor b cell Plasmablast Germinal center b cell Plasma cell B cell

Thoracic lymph node Bone marrow Jejunum lamina propria Epithelial lining fluid Spleen Inguinal lymph node Jejunal epithelium Blood Mesenteric lymph node Lung

64-year-old stage 68-year-old stage Fifth decade stage 73-year-old stage 75-year-old stage 25-year-old stage Eighth decade stage Fourth decade stage 56-year-old stage 20-year-old stage 67-year-old stage Sixth decade stage 23-year-old stage 27-year-old stage 48-year-old stage 33-year-old stage Third decade stage

Normal

Female Male

10x 3' v3 10x 5' v2

Cell

Homo sapiens

Non-classical monocyte Lung megakaryocyte Natural killer cell Mature conventional dendritic cell Alveolar type 1 fibroblast cell Classical monocyte Pulmonary alveolar type 1 cell Regulatory t cell Secondary crest myofibroblast Mast cell Vascular associated smooth muscle cell Helper t cell Pericyte Alveolar adventitial fibroblast Alveolar capillary type 2 endothelial cell Tracheobronchial smooth muscle cell Ciliated cell Mucus secreting cell of tracheobronchial tree submucosal gland Vein endothelial cell Cd8-positive, alpha-beta cytotoxic t cell Vein endothelial cell of respiratory system Lung interstitial macrophage Brush cell of tracheobronchial tree Conventional dendritic cell Pulmonary alveolar type 2 cell Alveolar capillary type 1 endothelial cell Plasma cell Alveolar macrophage Goblet cell Pulmonary neuroendocrine cell Plasmacytoid dendritic cell Serous secreting cell Mesothelial cell of pleura Secretory cell Endothelial cell of respiratory system lymphatic vessel Endothelial cell of artery B cell Basal cell Lung secretory cell

Middle lobe of right lung

11-month-old stage 4-month-old stage 15-month-old stage 3-year-old stage 12-month-old stage 19-month-old stage 14-month-old stage 10-month-old stage 5-month-old stage 13-month-old stage 7-month-old stage 6-month-old stage 8-month-old stage 20-month-old stage 21-month-old stage 18-month-old stage

Normal Bronchopulmonary dysplasia

Female Male

10x 3' v3

Nucleus

Homo sapiens

Non-classical monocyte Natural killer cell Naive b cell Classical monocyte Cd8-positive, alpha-beta t cell Endothelial cell of lymphatic vessel Mast cell Fibroblast Igg plasma cell Pericyte Epithelial cell of proximal tubule Naive thymus-derived cd8-positive, alpha-beta t cell Collagen secreting cell Kidney collecting duct principal cell Naive thymus-derived cd4-positive, alpha-beta t cell Macrophage Gamma-delta t cell Epithelial cell of proximal tubule segment 1 Mhc-ii-positive classical monocyte Unswitched memory b cell Kidney loop of henle thick ascending limb epithelial cell Kidney pelvis urothelial cell Kidney connecting tubule principal cell Epithelial cell of proximal tubule segment 3 Class switched memory b cell Kidney distal convoluted tubule epithelial cell Kidney loop of henle ascending limb epithelial cell Epithelial cell Conventional dendritic cell Renal beta-intercalated cell Plasma cell Effector memory cd4-positive, alpha-beta t cell Renal alpha-intercalated cell Iga plasma cell Cd4-positive, cd25-positive, alpha-beta regulatory t cell Mucosal invariant t cell Plasmacytoid dendritic cell Activated type ii nk t cell Endothelial cell Innate lymphoid cell Cd4-positive, alpha-beta t cell Effector memory cd8-positive, alpha-beta t cell B cell Podocyte

Kidney Vein Adrenal tissue Blood Perirenal fat

Fifth decade stage Eighth decade stage Seventh decade stage Sixth decade stage

Normal Kidney oncocytoma Nonpapillary renal cell carcinoma Kidney benign neoplasm

Female Male

10x 5' transcription profiling

Cell

Homo sapiens

Retinal bipolar neuron Retinal cone cell H2 horizontal cell Gabaergic amacrine cell S cone cell Amacrine cell Rod bipolar cell Mueller cell Parasol ganglion cell of retina Retinal ganglion cell Retinal pigment epithelial cell Microglial cell Midget ganglion cell of retina Retinal rod cell H1 horizontal cell Starburst amacrine cell Glycinergic amacrine cell Astrocyte

Macula lutea proper Peripheral region of retina Chorioretinal region Retinal neural layer Fovea centralis

80-year-old stage 64-year-old stage 65-year-old stage 68-year-old stage 82-year-old stage Adult stage 42-year-old stage 86-year-old stage 54-year-old stage 74-year-old stage 89-year-old stage 53-year-old stage 78-year-old stage 69-year-old stage 60-year-old stage 71-year-old stage

Normal

Female Male

10x 3' v2 10x 3' v3

Cell

Homo sapiens

Natural killer cell Naive b cell Endothelial cell of lymphatic vessel Regulatory t cell Capillary endothelial cell Mast cell Monocyte Pericyte Enterocyte of epithelium of large intestine Gastrointestinal tract (lamina propria) macrophage of large intestine Paneth cell T-helper 17 cell Memory b cell Enteroendocrine cell Macrophage Gamma-delta t cell Central memory cd4-positive, alpha-beta t cell Glial cell Vein endothelial cell Tuft cell of colon Conventional dendritic cell Germinal center b cell Enterocyte Plasma cell Best4+ enterocyte Effector memory cd4-positive, alpha-beta t cell T-helper 1 cell Stromal cell Stem cell Transit amplifying cell Goblet cell Myofibroblast cell Follicular b cell Innate lymphoid cell T follicular helper cell Effector memory cd8-positive, alpha-beta t cell Endothelial cell of artery B cell

Ileum Large intestine Ascending colon Sigmoid colon Duodenum Descending colon Rectum Colonic epithelium Small intestine Caecum Transverse colon Jejunum Vermiform appendix

Fifth decade stage 29-year-old stage Adult stage Eighth decade stage 53-year-old stage Seventh decade stage 45-year-old stage Sixth decade stage Third decade stage

Normal

Female Male

10x 3' v2 10x 3' v3 10x 5' v2 10x 3' v1

Cell